8.2.4 Concentration 1/Diafiltration 1/Diafiltration 2/Concentration 2 Mode
C/D/D/C Mode may be used to wash the solution with two different buffers.
NOTE: In C/D/D/C Mode Auxiliary Pump 2 functions as Diafiltration Pump 2. For this functionality
test, prepare two buffer reservoirs that both lead into the Feed Reservoir, and have one line go
through Auxiliary Pump 1 and the other line go through the Auxiliary Pump 2.
Starting Weight 20 kg
Conc Factor 1 10X Perm Wt After C1 18 kg
Feed Wt After C1
2 kg
Diaf Volumes 1 7 Diaf Wt 1 14 kg
Perm Wt After D1 32 kg
Diaf Volumes 2 8 Diaf Wt 2 16 kg
Perm Wt After D2 48 kg
Conc Factor 2 20X
Feed Wt After C2
1 kg
Feed Scale Permeate Scale