REPLIGEN KrosFlo KR2i

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DOC0304

eRev. [Major Version]1.01.0 8/9/2023

User Guide

KrosFlo® KR2i Real-Time Process Management (RPM™) System

2. Click “Start” when the “Unloading Flow Cell” pop-up appears.

3. Follow the onscreen instructions and animations to unload a flow cell.

4. Upon completion of unloading the flow cell, the pop-up window will automatically close and return to the previous VPT

Device menu. The serial number and flow cell name will be removed automatically.

9.3 Software/Firmware Operation

VPT Devices operate through TFF System firmware and software; please contact Repligen Customer Service to ensure latest

firmware and software combination has been loaded on TFF System and PC prior to operation.

Main Page

Table of Contents3
Table 1. Explanation of User Attention Phrases7
Table 2. Safety Precautions for Krosflo Kr2I RPM System8
Table 3. Performance Specifications9
Table 4. Electrical Input Specifications9
Table 5. Physical Specifications10
Table 6. Environmental Specifications11
Table 7. Compliance Summary11
Table 8. Computer Specifications: Included Tablet12
Table 9. Computer Specifications: Minimum Requirements (if Supplied by User)12
Table 10. System Parts List13
Table 11. Materials of Construction14
Figure 1. Manual Mode Setup Configuration15
Figure 2. Concentration Mode (C. Mode) Setup Configuration16
Figure 3. C/D and C/D/C Mode Setup Configuration17
Figure 4. C/D/D/C Mode Setup Configuration17
Figure 5. CFC Mode Setup Configuration18
Figure 6. CF/D/C Mode Setup Configuration19
Table 12. ACR2-H3I-01N Typical Flow, Pressure, and Vacuum Data - 3 Roller Pumps21
Table 13. ACR2-H4I-01N Typical Flow, Pressure, and Vacuum Data - 3 Roller Pumps21
Figure 7. Attaching Mounting Plate to Drive22
Figure 8. Back of Research II Pump Head to Drive22
Figure 9. Position for Engaging Bayonet Feature for Horizontal Mounting22
Figure 10. Bayonet Feature Locked in Horizontal Pump Orientation23
Figure 11. Pump Head in Fully Closed Position23
Figure 12. Pump Head in Fully Open Position24
Figure 13. Tubing Path through Pump Head-During Loading24
Figure 14. Preparation to Mount a Second Pump Head25
Figure 15. Engaging Bayonet of Second Pump Head to Bayonet Tabs on First Pump Head25
Figure 16. Drive with both Pump Heads Locked in Position First Pump Head25
Connecting the Flowvpx Head to the Flowvpx Standard Mount26
Figure 17. Ctech Flowvpx System: Body26
Figure 18. Flowvpx Head, Logo Side down26
Figure 19. Standard Mount Clamp on Flowvpx Head27
Figure 20. 5 MM Ball End Driver27
Figure 21. Clamp Handle Installation27
Figure 22. Rotate Clamp Handle27
Figure 23. Tighten Mounting Post Clamp Handle28
Figure 24. Install Flowvpx Head on Mounting Post28
Figure 25. Lower Flowvpx Head on Mounting Post28
Connecting the Flowvpx Instrument to the Cary 60 Spectrophotometer and Computer29
Figure 26. Tighten Standard Mount Clamp Handle (Left) and Repositioning the Clamp Handle (Right)29
Figure 28. Accessory Port in Back of Cary 60 Spectrophotometer29
Figure 29. Detector Cable in Sample Compartment30
Figure 30. Cary 60 Power Cable and USB Cable, Back Panel30
Figure 31. Fiber Optic Coupler Threaded Splice Bushing30
Figure 32. Flowvpx I/O Cable Connections31
Figure 33. Connect Power and I/O Splitter Cable (EC0208) to Flowvpx Head31
Table 14. ACR2-H4I-01N Typical Flow, Pressure, and Vacuum Data - 3 Roller Pumps32
Figure 34. I/O External Cable (EC0214) to Flowvpx Head32
Figure 35. USB Cable (EC0207) to Flowvpx Head32
Loading the Flow Cell33
Figure 36. Flowvpx USB Cable (EC0207) to Computer33
Unloading the Flow Cell35
Installation37
Figure 36. KR Jr Display in Remote Control Mode37
Auxiliary Pump Settings38
ABV Installation38
ABV Settings38
Table 15. KR Jr Tubing Specifications38
Figure 37. Automatic Backpressure Valve38
System Configuration39
Basic Setup39
Figure 38. Back of Konduit, Green Power Indicator Light39
Maintenance40
Figure 39. Front of Konduit40
Table 16. Troubleshooting43
Table 17. Replacement and Auxiliary Parts Information44

Table of Contents

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