3
Section 2
Section 2Section 2
Section 2
Guidance Notes and Routine Checks
2.1 To ensure that chamber conditions remain as stable as possible always minimize the length
of time that the inner door is open. The magnetic door catch is specifically designed to make door
opening and closing as easy as possible. When opening the inner door, wipe off any small drops of
condensate that may have formed on the inner seal. This will avoid a build up of condensation.
2.2 For cell culture work – we recommend the use of copper sulphate (or recognised biocide) in
the humidity tray. Tests have shown that as well as inhibiting bacterial growth in the tray this
can reduce contamination on the chamber walls (one small teaspoonful copper sulphate
recommended).
2.3 For IVF and other sensitive work – we do not recommend the use of any biocide in the
humidity tray. To reduce the possibility of contamination, the humidity tray water should be
changed and the humidity tray cleaned with 70% Alcohol / 30% distilled water solution every
10 – 14 days. To remove / replace the humidity tray: - turn the tray through 45° and remove
for refilling. To replace the humidity tray, carefully turn through 45° and pass the tray through
the front of the shelf racks.
2.4
Daily
2.4.1 Check that the temperature and CO
2
levels are reading within specification.
2.4.2 Check the reserve pressure in the CO
2
cylinder (normally 50 bar when full). The design of
the incubator ensures very low consumption of CO
2
and during normal working conditions a type K
cylinder should last approximately 12 months. If there is a significant drop at the cylinder 50 bar
pressure, it means that the cylinder is almost empty and should be replaced, ensuring that there are
no leaks at any of the joints.
2.5
Monthly
2.5.1 If not changing the humidity tray water / cleaning humidity tray regularly - top up the humidity
tray (to a maximum volume of 0.5 litres). The use of warm water (approx. 37.0°C) will ensure a rapid
return to optimum chamber conditions. After topping-up, check that the humidity tray is central
between the racks and pushed back against the stops.