RS Mini Galaxy E 050-600

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Summary

Guidance Notes and Routine Checks

Daily Checks

Perform daily checks on temperature, CO2 levels, and cylinder pressure.

Monthly Checks

Top up the humidity tray monthly and ensure its placement.

Installation

Unpacking, Components, and Requirements

Details unpacking, components, electrical, gas, and accessory needs.

Positioning and Shelf Rack Assembly

Guidance on positioning, fitting feet, and assembling shelf racks.

Connecting CO2 Supply

Instructions for connecting the CO2 supply tubing and ensuring no leaks.

Initial Power Up and Setup

Connecting power, initial conditions, and humidity tray setup.

Setting the Humidity Conditions

Humidity Tray Setup and Biocide Use

Filling the humidity tray with water and recommended biocides for cell culture.

Contamination Prevention

Advice on changing water and cleaning the tray for sensitive work.

Sensor Protection Warning

Warning about leaving water in the tray when the incubator is off to protect the CO2 sensor.

Control Panel Description

Control Panel Layout and Keys

Describes the two LED displays and four keys on the control panel.

Display Indicators

Explains how flashing decimal points indicate the alarm system is not armed.

Programming Temperature, CO2 & Auto Zeroing

Programming Temperature and CO2 Levels

Details the ranges and steps for programming temperature and CO2 levels.

CO2 Auto Zero Procedure

Explains the purpose and monthly recommended procedure for CO2 sensor auto-zeroing.

Auto Zero Operational Steps

Step-by-step guide for the CO2 Auto Zero process including door actions and countdowns.

Programming The Alarm System

Setting Temperature and CO2 Alarms

How to set high and low temperature and CO2 alarm limits.

Door Open Alarm Settings

Configuring the delay time before the door open alarm sounds.

Alarm Duration and Arm Time

Setting how long alarms are active and the time for conditions to recover.

The Alarm System

Alarm Activation Logic

Explains when the alarm system is armed, triggered by deviations from set points.

Door Opening and Alarm Arming

How opening the door affects the alarm system and the arm timeout.

Alarm Acknowledgement and Cancellation

How alarms are cancelled and messages are handled after conditions recover.

System Alarms

Temperature Sensor Alarms

Describes messages and behavior when temperature sensors fail or recover.

Over Temperature Cut-out; Alarm

Details the two modes of operation for the over-temperature safety system.

CO2 Auto Zero Alarms

Explains CO2 alarms related to the Auto Zero function and sensor defects.

Cleaning and Disinfecting

External Cleaning

Basic instructions for cleaning the incubator's exterior.

Disinfection and Internal Cleaning Procedures

Steps for disinfecting and cleaning internal components, including sensor protection.

Prohibited Cleaning Agents

Lists substances that should never be used for cleaning to avoid damage.

Technical Specification

Temperature Management Specifications

Details temperature range, control, stability, and uniformity.

CO2 Control Specifications

Outlines CO2 range, control, stability, uniformity, and gas requirements.

Relative Humidity Specifications

Information on humidity reservoir capacity and control levels.

Shelves Specifications

Details shelf material, dimensions, and configuration options.

Electrical Supply Requirements

Storage Temperature Guidelines

Ordering Information

Base Model Numbers

Lists base model numbers for different voltage/frequency configurations.

Factory Fitted and Model Options

Details no-charge options and additional optional features available.

Accessories

Shelves, Stands, and Software

Lists available shelves, floor stands, and data collection software.

CO2 Related Accessories

Lists accessories like regulators, filters, and gas analyzers.

The RS Biotech Mini Galaxy E CO2 Incubator is a laboratory device designed for precise and reliable cell culture, ensuring stable chamber conditions for optimal growth. It features a microprocessor control system that facilitates a rapid and controlled return to set conditions after door openings, preventing temperature overshoot. The incubator is equipped with a touch-sensitive keypad and two 3-digit LED displays for easy setting and monitoring of chamber parameters.

Function Description:

The primary function of the Mini Galaxy E is to provide a controlled environment for cell culture, specifically regulating temperature and CO2 levels. It incorporates a thermal heating element that completely surrounds the incubator, ensuring even temperature distribution within the chamber. An independently heated outer door prevents condensation on the inner door. An over-temperature safety system operates independently of the main control system for enhanced safety. The humidity control system is designed to maintain high uniform relative humidity and prevent condensation, with a manual adjustment range of approximately 95-97% r/h. CO2 levels are controlled by a solid-state infrared sensor, which is reliable and unaffected by humidity. The CO2 system includes a semi-automatic zero function for maintaining accurate CO2 levels. A two-level alarm system alerts users to chamber condition deviations or system faults, with a programmable chamber monitoring alarm that can be disarmed if not required. Air circulation is achieved without a fan, reducing contamination risk and sample evaporation.

Important Technical Specifications:

Temperature Management:
- Digital programming via microprocessor control in 0.1°C increments.
- Measurement of chamber and door temperature via 4 RT matched thermistors (sensitivity 0.01°C).
- Adjustable independent control of door heater.
- "Out of Limits" temperature protection system independent of microprocessor control.
- Range: 4°C above ambient temperature to 50°C.
- Control: ± 0.1°C.
- Stability: ± 0.1°C.
- Uniformity: ± 0.2°C.
CO2 Control:
- Solid-state infra-red CO2 sensor operating independently of humidity.
- Programmable, fully automatic zeroing facility.
- Range: 0.2 - 10%, or 0.2 - 20% (with optional CO2 control).
- Control: ± 0.1%.
- Long Term Stability: ± 0.2%.
- Uniformity: ± 0.1%.
- Recovery Rate: Better than 0.7% per minute.
- Gas Connections: 6mm tubing.
- Required Gas Pressure: 0.35 bar / 5psi.
Relative Humidity:
- Completely dry wall chamber with removable stainless steel humidity reservoir.
- Reservoir Capacity: 0.5 litres.
- Humidity Control: Normal: 95% at 37°C; High: 97% at 37°C.
Shelves:
- Polished stainless steel, non-perforated.
- Usable Space (WxDxH): 350 x 250 x 100mm per shelf (with 3 shelves).
- Usable Area: 875cm² per shelf.
- Standard No. of Shelves: 3.
- Multiple Position Option: 2 to 6 shelves.
Alarm Systems:
- Two-level alarm system with programmable audio/visual warnings.
- Level 1 signals system failures, Level 2 monitors chamber conditions.
Dimensions:
- Chamber (HxWxD): 401 x 401 x 308mm.
- Chamber Volume: 48 litres.
- External (HxWxD): 645 x 484 x 470mm.
- Crated Dimensions (HxWxD): 790 x 620 x 610mm (including pallet).
Weight:
- Uncrated: 32kg (approx – actual weight depends on options fitted).
- Crated: 50kg.
Electrical Supply:
- Earthed Electrical Supply: 220 - 240V 50/60Hz or 100-120V 50/60Hz.
- Power: 500 watts.
- Energy to maintain 37°C: < 0.1kwh.
Storage Temperature: 10-50°C.

Usage Features:

Easy Setting and Monitoring: Touch-sensitive keypad and LED displays allow for straightforward parameter adjustment and real-time monitoring.
Rapid Recovery: Microprocessor control ensures quick return to set conditions after door openings.
CO2 Auto Zero: A semi-automatic zero function references the CO2 sensor to atmospheric CO2 (0.05%) monthly, maintaining accuracy. This involves opening and closing the inner door as per displayed instructions.
Alarm Programming: Users can program high and low temperature and CO2 alarms, adjust door open alarm delay, and set alarm duration and arm time.
Humidity Tray: The humidity tray is easily removed and replaced for refilling and cleaning. Warm distilled water (approx. 37.0°C) is recommended for rapid return to optimum conditions.
Contamination Control: For cell culture, copper sulphate or recognized biocide is recommended in the humidity tray. For IVF and sensitive work, regular cleaning with 70% Alcohol / 30% distilled water solution is advised.
Installation: Requires an earthed electrical supply and a CO2 gas cylinder with a two-stage regulator. Care should be taken to avoid draughts, direct sunlight, or proximity to radiators.
Shelf System: The shelf rack system is designed for complete removal, providing easy access to the smooth interior for cleaning.

Maintenance Features:

Daily Checks: Verify temperature and CO2 levels are within specification and check the reserve pressure in the CO2 cylinder.
Monthly Checks: Top up the humidity tray if not regularly changed/cleaned. Ensure the tray is central and pushed back against the stops.
Cleaning and Disinfecting:
- Exterior: Wipe with a soft cloth and soapy water.
- Interior: Recommended disinfecting agent is 70% Isopropanol (alcohol) and 30% distilled water.
- Procedure: Program 0.0% CO2, switch off, disconnect from mains. Wipe external surfaces. Remove shelves, humidity reservoir, shelf runners, shelf racks, and inner door seal. Place protective cover over the CO2 sensor to prevent damage from disinfectant. Clean humidity reservoir with sterile water and alcohol solution. Wipe inside of the chamber and internal components twice with alcohol/water solution, allowing to dry completely. Re-assemble and wipe inner door seal.
- Post-Cleaning: Refill humidity tray, allow incubator to stabilize for at least two hours (preferably overnight), then perform an Auto Zero and re-program the desired CO2 level.
- Caution: Never use Sodium Azide, Aqua Regia, Iodine, Ferric Chloride, or Sulphuric Acid to clean stainless steel as it will cause damage. Do not leave water in the humidity tray if the incubator is switched off, as this may damage the CO2 sensor.
Sensor Protection: Protective covers for the CO2 sensor (and optional Oxygen/humidity sensors) must be used during cleaning to prevent damage from disinfectant.