Awareness Technology, Inc. Stat Fax 303 Plus Operator’s Manual Rev. J 27
Enter a number which, when multiplied by the cutoff value, will be the upper limit of the Negative
range (enter 1 if you want Negative values to be all values less than the cutoff value). The
display will prompt “POS>=X*COV; X=”. Now enter a number which, when multiplied by the
cutoff value, will be the lower limit of the Positive range (enter 1 if you want Positive values to be
all values greater than or equal to the cutoff value). Any samples with absorbances falling
between these ranges will be labeled “Equivocal”.
The instrument then asks for the number of each type of control to be read. You may select 0 for
# of Neg Controls and/or Pos Controls, but you must choose at least one Cutoff Control. The
instrument will also ask for the absorbance acceptance criteria for the mean of each type of
control.
For each type of control, the instrument asks for the number of each type of control to be read,
the absorbance acceptance criteria, and if the criteria apply to the individual wells or the mean of
the wells for that control.
The display will show “Num. of NCs =”. Type in the number of Negative Controls and press
ENTER.
The display will show: “Neg C. <=”, “Neg C. >=”. Type in each value and press ENTER.
If you select 2 or more wells for each control the display will prompt “Check mean Y/N”. Press
NO if the criteria apply to the individual wells or YES if the criteria apply to the mean of the wells.
The display will show: “Num. of CCs =”. Type in the number of Cutoff Controls and press
ENTER.
The display will show: “C/O C.>=”, “C/O C.<=”. Type in each value and press ENTER.
The display will show: “Num. of PCs=”. Type in the number of Positive Controls and press
ENTER.
The display will show: “Pos C. >=”, “Pos C. <=”. Type in each value and press ENTER.
The instrument will then prompt “Corr Factor=Y/N”. This correction factor is multiplied by the
mean of the C/O Controls to result in the actual cutoff value. Press NO if you do not want to use
a correction factor. Press YES and the display will show “Corr Factor=”. You may enter a
correction factor with up to 3 decimal places.
The display will show: “Done Y/N”. Press YES to run the test. Press NO to select options for
additional quality control values. You may chose to check the ratio or the difference between the
means of the positive and negative controls to assure adequate distinction. The display will
show: “Ratios Y/N”. Press YES to enter the ratio value for “mP/mN >=”. Enter the lowest
acceptable ratio for the mean of the positive controls to the mean of the negative controls. Press
NO and the display will show “Difference Y/N”. Press YES and the display will show “mP-mN
>=”. Enter the lowest acceptable difference for the mean of the positive controls minus the mean
of the negative controls.
Many options not required can be bypassed by pressing ENTER. Otherwise, type each value
and press ENTER. Once all information has been entered, “Set Carrier to X”, “Then Press
Enter” is displayed, where X is either 1 or A, depending on the strip type. Load the first strip into
the right-most carrier position and press ENTER. While the strip is being automatically
positioned for reading, the display will indicate “Positioning Strp”.
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