Awareness Technology, Inc. Stat Fax 303 Plus Operator’s Manual Rev. J 29
The instrument will then prompt you to enter the calibrator values. As you enter the values, the
printer will print:
“CAL 1= XXXX”
“CAL 2= XXXX”
“CAL 3= XXXX”.....
where XXXX represents the calibrator values you entered. Except for the log/logit mode, which
requires increasing calibrator values and decreasing calibrator absorbance, the points may be
entered in any order, but must be read in that same order for accurate pairing. The regression
line may be used for both positive and negative slopes. The number of standards must be less
than or equal to eight (seven with a blank).
NOTE: When using a format which calculates the ln of the concentration, do not use 0.0
for the concentration, since ln 0 is not defined. Likewise, when using ln of 1000*Abs, the
absorbance values must be non-zero and positive.
Note that the instrument allows calibrator values up to 999999, and calibrators less than 1000
can have up to two places after the decimal point. Decimal places for the reported
concentrations will be the same as those selected for calibrator 1.
After all the calibrator values have been entered, the display will prompt “Off Curve Ok Y/N”.
Press YES if all samples are valid. Press NO to flag all samples having absorbance values
higher than the highest calibrator or lower than the lowest calibrator. Note that the “Off Curve”
flagging feature requires that the calibrators be entered and read from lowest concentration for
calibrator #1 to highest concentration for the final calibrator.
“Set Carrier to X”, “Then Press Enter”, where X is either 1 or A, depending on the strip type.
Load the first strip into the right-most carrier position and press ENTER. While the strip is being
automatically positioned for reading, the display will indicate “Positioning Strp”.
After the strip or strips containing the standards are read, there will be a pause after the standard
curve is read. Next, the slope and intercept of the linear regression will be printed. The
correlation coefficient (r) will also be given as an aid in determining the acceptability of the curve.
The display will prompt “PLOT CURVE Y/N”. To plot the regression line press YES. Press NO to
continue without printing the curve.
If calibrators were read in duplicate, you may choose to edit the curve. The display will prompt
“DELETE WELLS Y/N”. Use the YES or NO key to answer. If you press NO, the instrument will
proceed to read and calculate the remainder of the strips. If you choose to edit the curve by
pressing the YES key, the display will ask for the location of the well(s) that are to be deleted.
Enter the wells one by one. You may delete as many as one from each pair, but you may not
delete an entire point. When you have finished deleting wells, press the ENTER key again. The
standard curve will be recalculated showing an “X” beside the deleted well(s). If one of a
duplicate pair is deleted, the remaining point will be considered as the value for that standard.
The new slope, intercept, and correlation coefficient will be printed. Again the operator will have
an opportunity to see the graph and to edit. This may be repeated as many times as desired until
either an acceptable curve is obtained, or the mode is canceled. To continue reading specimens,
answer NO to the question “DELETE WELLS Y/N”.
The instrument will be ready for the next strip when it displays “Set Carrier”, “Then Press
Enter”. To continue reading in the Regression mode, load the next strip and press the ENTER
key. Press CLEAR twice to cancel the mode.
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