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CyFlow Ploidy Analyser
SYSMEX CyFlow Ploidy Analyser Instrument Operating Manual
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Sysmex Partec GmbH
Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · info@sysmex-partec.com ·
www.sysmex-partec.com
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Revi
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Rev-
03_201
4-
10
-
22
Issued by:
Sysmex
Partec GmbH
2
Table of Contents
Table of Content
3
Table of Contents
3
1 Introduction
5
2 Presentation
6
Basics
6
What Are Topics Covered by this Manual
6
What Other Manuals Are Available
6
What Should I Know before Operating the Cyflow
6
Ploidy Analyser
6
Introduction to Flow Cytometry
7
Flow Cuvette and Light Signals
7
Optical Standard Setup - Parameters and Filters
8
In Flow Cytometry, What Is
10
A Parameter
10
A Histogram Channel
10
The Count in a Histogram
10
A Peak
10
Background in a Histogram
10
3 Instrument Starting Procedure
11
Sheath Fluid Level Control And/Or Refill
11
Switching on the Cyflow
11
User Levels
13
4 Cyview TM Controls
14
Cyview™ Instrument Setting Controls
15
2D PLOTS- Display Options
15
Results
16
Regions
17
GENERAL - Properties of the Instrument
18
ACQUISITION - Instrument Control
18
MEASURE - Definition of Measure Modes
19
Measure Modes
20
5 Cyview TM Instrument/Measurement Settings
23
Priming / Initialization Process
23
Work Process
25
Shut down Process
25
Intermediate Cleaning Process
26
Sheath and Waste Bottle
27
6 Measurement Parameters
27
Instrument Setting Properties
27
Threshold Settings (T)
27
Flow Speed (S)
27
Light Source (L)
28
2D Plot Properties
28
Region/Roi Properties
29
Compensation
31
7 Typical Sample Analysis
32
8 Automatic Peak Analysis
34
MAIN USER/USER Mode
34
SERVICE/ADMINISTRATOR Mode
36
Export of Peak Analysis and 1-Parameter Histograms
37
9 Keyboard/Mouse Combinations
39
10 Appendix
40
Biohazards
40
Maintenance
41
Service
41
Transport and Storage
42
Disposal
42
Laser Safety
43
Technical Specifications
44
Optical Standard Setup
44
Cyflow ® Ploidy Analyser System
44
Cyflow ® Ploidy Analyser Optics
45
11 Notes
46
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SYSMEX CyFlow Ploidy Analyser Specifications
General
Brand
SYSMEX
Model
CyFlow Ploidy Analyser
Category
Measuring Instruments
Language
English
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