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SYSMEX SF-3000 - User Manual

SYSMEX SF-3000
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SF-3000/06.95
OPERATOR’S MANUAL
AUTOMATED HEMATOLOGY ANALYZER
SF-3000
SYSMEX CORPORATION
KOBE, JAPAN
Copyright © 1995 - 2001 by SYSMEX CORPORATION
All rights reserved. No part of this Operator’s Manual may be Code No. 461-2459-3
reproduced in any form or by any means whatsoever without PRINTED IN JAPAN
prior written permission of SYSMEX CORPORATION. Date of Last Revision: March 2001

Table of Contents

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SYSMEX SF-3000 Specifications

General IconGeneral
BrandSYSMEX
ModelSF-3000
CategoryMeasuring Instruments
LanguageEnglish

Summary

Chapter 1: Introduction

1. Introduction to the SF-3000 Manual

Introduces the SF-3000 analyzer, its capabilities, and the manual's structure.

2. Operators Manual Organization

Explains the manual's chapter organization and content for efficient information retrieval.

3. Informational Headings and Document Conventions

Details the use of NOTE, CAUTION, and WARNING boxes and document conventions.

4. System Overview

Introduces the SF-3000 analyzer's main elements and options.

5. System Options

Lists optional features to enhance system efficiency like bar code readers and printers.

6. Safety Precautions

Covers crucial safety considerations for operating, maintaining, and servicing the SF-3000 analyzer.

7. Operational Summary

Summarizes operating procedures for different modes, screen displays, and touch panel functions.

8. Analysis Parameters

Lists the 23 parameters analyzed by the SF-3000, their acronyms, and detection methods.

9. Display Screen Layout and Functions

Explains the layout and components of the LCD screen, including status and menu areas.

10. Password Protection System

Explains the password system for protecting QC, Settings, and Calibration programs.

11. Emergency Shutdown Procedure

Details the procedure for shutting down the analyzer in an emergency situation.

12. System Alarms and Notifications

Describes the three types of alarm sounds used to alert the operator.

13. Installation Requirements

Outlines necessary space, electrical, and environmental requirements for installation.

14. System Specifications

Provides detailed technical specifications for the SF-3000 system.

Chapter 2: Sample Processing

1. Introduction to Sample Processing Modes

Introduces sample processing modes: manual open, capillary, auto (sampler), manual closed, and manual CP.

2. Start-Up Procedures and Operator Checks

Outlines essential operator checks before starting the SF-3000 system, including reagents and equipment.

3. Quality Control Analysis Procedures

Explains how to perform QC analysis in Manual Mode, including sample number setting and QC file specification.

4. Analysis Mode Procedures

Details the operational steps for Manual Open, Capillary, Auto, Manual Closed, and Manual CP modes.

5. Shutdown Procedure

Details the shutdown sequence for cleaning detector chambers and manometers.

6. Timer Mode Operations

Explains the timer mode for automatically switching off the pneumatic unit power after inactivity.

Chapter 3: Work List Management

1. Introduction to Work List Functionality

Introduces the Work List function for specifying samples and processing analysis information.

2. Executing a Work List Program

Details how to input analysis information using the Work List display and its second menu.

3. Downloading Work List Data

Details how to download order information from a host computer or floppy disk.

4. Deleting Work List Entries

Explains how to delete specified order information from memory, including current, marked, or all data.

5. Graphic Printer (GP) Output

Describes how to print the list of order information to a Graphic Printer.

6. Backing Up Work List Data

Explains how to copy registered order information to a floppy disk for backup purposes.

Chapter 4: Result Interpretation and Reporting

1. Introduction to Result Analysis

Introduces the chapter on analysis results, screen displays, output, and processing.

2. Interpretive (IP) Messages Guide

Explains the system's IP messages, their meanings, and conditions for appearance.

3. Stored Data List Display

Explains how stored analysis data is displayed in list format and how to select data types.

4. Graphic Display Options

Describes how to display scattergrams and particle size distributions for analyzed data.

5. Data Validation Procedures

Outlines the process for reviewing and validating data for report output.

6. Marking Data for Output;Deletion

Explains how to mark data in the List Display with a yellow square for output or deletion.

7. Selecting Data for Display

Allows the operator to select the type of samples to be displayed in the List Display.

8. Searching Stored Data

Allows specifying data to display at the top or bottom of the List Display.

9. Sorting Stored Data

Arranges List Display data chronologically or by Sample ID order.

10. Editing Sample Information

Allows changing sample information of stored analysis data.

11. Outputting Stored Data

Explains how to output specified stored data to printers or host computer.

12. Deleting Stored Data

Explains how to delete specified stored data from the List Display.

13. Auto Output Configuration

Explains how SF-3000 automatically prints or transmits analysis data based on preset conditions.

Chapter 5: Maintenance and Supplies

1. Introduction to Maintenance

Emphasizes periodic maintenance for keeping the SF-3000 in optimal operating condition.

2. SF-3000 Maintenance Check List

Provides a periodical maintenance checklist for daily, weekly, monthly, yearly, and as-needed tasks.

3. Daily Maintenance Procedures

Details daily maintenance procedures, including cleaning detector chambers and removing fluid from the pneumatic unit trap chamber.

4. Weekly Maintenance Procedures

Outlines weekly maintenance tasks, including cleaning the SRV tray and executing the WBC detector cleaning sequence.

5. Monthly Maintenance Procedures

Covers monthly maintenance tasks like cleaning the orifice, waste chamber, and sample rotor valve.

6. Yearly Maintenance Procedures

Describes the annual visual inspection for laser safety.

7. As-Needed Maintenance Procedures

Details maintenance procedures required based on specific conditions or errors, such as cleaning mechanisms or removing clogs.

8. Replacement Procedures

Lists procedures for replacing consumables like reagents, piercers, fuses, and belts.

9. Resetting System Counters

Explains how to reset system unit operation counters after maintenance.

10. Formatting Floppy Disks (FD)

Provides instructions for formatting a 3.5-inch floppy disk for SF-3000 use.

11. Supplies and Replacement Parts

Lists all supplies and replacement parts with their part numbers and reference sections.

Chapter 6: Quality Control (QC)

1. Introduction to Quality Control

Explains the purpose of Quality Control (QC) in checking analysis data accuracy and precision.

2. Deleting QC Files

Describes how to delete existing QC data from memory for new lots or level changes.

3. System Settings for QC

Covers setting the control method (X or L-J) and limit display method for QC.

4. QC Parameter Selection

Allows excluding unnecessary parameters from X/L-J and X M QC analysis.

5. Setting X M Control Parameters

Explains how to start/stop X M Control and set the number of samples per batch.

6. Target and Limit Value Configuration

Used for manually setting or downloading QC target and limit values.

7. QC Chart Display and Operations

Displays QC charts for data output, statistical calculations, and data point deletion.

8. Manual QC Analysis Procedures

Details how to perform QC analysis in Manual, Closed, or Manual CP modes using control blood.

9. Sampler QC Analysis (L-J Mode)

Explains how L-J Control can be performed in Auto Mode using control blood.

10. QC Methods and Applications

Covers commercial control applications (X, L-J) and patient sample analysis (X M).

11. Variable Target Function

Calculates control target values automatically from stored data.

12. Control Chart Definition

Defines how QC charts are read using the Levy-Jennings Method and how abnormalities are displayed.

Chapter 7: Calibration Procedures

1. Introduction to Calibration

Explains calibration purpose for compensating system biases to maintain accuracy.

2. HGB;HCT Automatic Calibration

Details the automatic calibration procedure for HGB and HCT using reference samples.

3. HGB;HCT Manual Calibration

Explains how to calibrate HGB and HCT values manually by entering updated values.

4. Calibration History Management

Guides on accessing, deleting, and outputting calibration histories.

Chapter 8: Troubleshooting Guide

1. Introduction to Troubleshooting

Introduces the chapter on error messages, countermeasures, maintenance, status displays, and error logs.

2. Troubleshooting by Error Messages

Provides an alphabetical index of error messages and a troubleshooting guide for problems encountered.

3. System Test Programs

Details test programs for checking system operation and identifying causes of abnormalities.

4. Status Display Interpretation

Explains how to check the system status, including analyzer condition, process status, and sensor status.

5. Error Log Management

Describes how to display past errors and recovery messages for corrective actions.

Chapter 9: Adjustment Procedures

1. Introduction to Adjustments

Explains that certain components of the SF-3000 system may require adjustment.

2. Pressure and Vacuum Display Monitoring

Details how to check and adjust pneumatic unit and main unit pressures and vacuum levels.

3. Pneumatic Unit Adjustment

Provides procedures for adjusting pneumatic unit pressure and vacuum levels.

4. Main Unit Adjustment Procedures

Covers adjusting main unit pressure and vacuum to specific levels.

5. LCD Contrast Adjustment

Explains how to adjust the contrast of the LCD screen using the control knob.

6. ID Bar Code Reader Adjustment (Optional)

Provides instructions for adjusting the ID Bar Code Reader, including installation and testing.

Chapter 10: Functional Descriptions

1. Introduction to Functional Descriptions

Explains detection principles, analysis methodology, procedures, and hardware components of the SF-3000.

2. Detection Principles Explained

Describes flow cytometry by semiconductor laser and DC detection methods for blood cell analysis.

3. Sample Flow Block Diagrams

Illustrates the sample flow for WBC and RBC/PLT/HGB analyses.

4. RBC;PLT;HGB Analysis Procedures

Explains the analysis flow for Red Blood Cells, Platelets, and Hemoglobin.

5. White Blood Cell (WBC) Classification

Details the classification of white blood cells using flow cytometry.

6. WBC Scattergrams Explained

Explains how scattergrams show 2D distributions of cell size and intracellular density.

7. RBC;PLT Particle Size Distribution Analysis

Covers analysis of RBC and PLT particle size distribution, including RDW and PDW.

8. Main Unit Electronic System

Describes the electronic system of the Main Unit and its microprocessor control.

9. Main Unit Component Identification

Identifies and describes components on the Main Unit's front, rear, sides, and interior.

10. Pneumatic Unit Components

Identifies and describes components of the Pneumatic Unit, including gauges, regulators, and switches.

11. Cap Piercing Sampler Unit (Optional)

Details the components and functions of the optional Cap Piercing Sampler Unit.

12. Manual CP Unit (Optional)

Describes the components and function of the optional Manual CP Unit.

13. Reagent System Overview

Explains the reagent system, including Diluent, Lyse Reagents, and Detergent.

Chapter 11: System Setup and Configuration

1. Introduction to System Setup

Explains that system setup is performed by the Sysmex Service Representative but can be modified by the user.

2. Auto Management Programs Configuration

Covers setting conditions for Auto Output, Auto Erase, and Auto Validation for peripheral devices.

3. Data Criteria Settings

Allows setting criteria for marking abnormal data (Mark Limits, Critical Limits) and stopping auto mode analysis (Stop Limits, Stop Conditions).

4. Flag System Configuration

Enables setting limits for Abnormal IP Messages for WBC, RBC, and PLT.

5. Peripheral Configuration Settings

Sets printer output conditions, print formats, and Host Computer interface parameters.

6. General System Setup Options

Allows setting system status such as Date/Time, Units, Password, Timer Mode, Normal Range, etc.

7. Output Set Values Configuration

Programs to print settings to a Graphic Printer and copy setting data to a floppy disk.

Appendix A: Menu Tree Navigation

ROOT MENU Structure

Shows the top-level menu structure of the SF-3000 system.

Sysmex Main Menu Options

Lists the main menu options accessible from the Sysmex key.

Sampler Unit Menu Options

Lists the menu options related to the Sampler unit.

Appendix B: Installation Guide

1. Preinstallation Checks and Considerations

Covers verifying box contents, power, and environmental considerations before installation.

2. Layout and Work Space Requirements

Specifies the recommended space around the instrument for servicing and heat dissipation.

3. Removing Packing Material

Details procedures for removing packing material from the front cover, front panel, and main unit chassis.

4. Tray Installation

Instructions for installing the supplied tray.

5. Bar Code ID Reader Installation (Optional)

Guides on attaching the ID Unit to the Main Unit chassis and connecting its wire cord.

6. CP Sampler Unit Installation (Optional)

Details the installation of the CP Sampler Unit, including removing covers, mounting metals, and connecting tubes.

7. Manual CP Unit Installation (Optional)

Covers the installation of the Manual CP Unit, including attaching dummy covers and grounding wires.

8. Tube Connection Procedures

Explains how to connect pneumatic unit vacuum and pressure lines, and reagent lines.

9. Battery Backup Installation

Describes the procedure for installing the battery backup PCB.

10. Cable and Power Cord Connections

Details connecting host computer, printers, and pneumatic unit power.

11. Power On and System Setup Procedures

Outlines procedures for initial power-on and system setup, including system checks.

Appendix C: Technical Information

1. Serial Interface for Host Computer

Details the serial interface specifications for connecting to a host computer, including connection and pin assignments.

2. Text Data Formats

Describes the formats for patient sample data, QC data, and inquiry data transmission.

3. ID Bar Code Specifications

Covers applicable bar codes, their elements, wide/narrow ratio, optical requirements, and check-digit methods.

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