SYSMEX SF-3000 User Manual

FUNCTIONAL DESCRIPTIONS
Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-9
5.2 WBC/BASO Analysis
Basophil determination and WBC number counting are performed in the WBC/BASO
channel using the flow cytometry. The following illustrates the procedure.
SRV
Reaction
Chamber
(Blood Sample : 33.0 µL)
WBC Detector
(Flow Cell)
CELLPACK
STROMATOLYSER-FB
: 0.967 mL
Figure 10-9: WBC/BASO Sample Flow
(1) The whole blood is aspirated from the whole blood aspiration tube to the sample
rotor valve.
(2) The 33.0 µL sample (measured by the sample rotor valve) is transferred to reaction
chamber. At the same time, 0.967 mL of reagent (STROMATOLYSER-FB) is
added to make 1:30 dilution.
(3) During the approximately 31.5-second interval in the reaction chamber, the red
blood cells are hemolyzed.
(4) The sheath injector piston slowly sends 30.4 µL of 1:30 diluted sample to the
WBC/BASO detector; then the system, using the flow cytometry by means of
semiconductor laser, performs the analysis.

SYSMEX SF-3000 Specifications

General

Brand	SYSMEX
Model	SF-3000
Category	Measuring Instruments
Language	English