FUNCTIONAL DESCRIPTIONS
10-8 Sysmex SF-3000 Operator's Manual -- Revised September 1995
5. WBC CLASSIFICATION
White blood cells (WBC) are generally classified as lymphocytes, monocytes, and
granulocytes. Granulocytes are further subclassified as neutrophils, basophils,
eosinophils. The SF-3000 uses the optical detector block incorporating the
semiconductor laser to analyze the WBC population. This section explains the applicable
analysis procedures.
5.1 4DIFF Analysis
In the DIFF channel, lymphocytes, monocytes, eosinophils and a group of neutrophils
and basophils are analyzed to determine the WBC 4-differentials using the flow
cytometry. The following illustrates the procedure.
SRV
Reaction
Chamber
(Blood Sample : 33.0 µL)
WBC Detector
(Flow Cell)
STROMATOLYSER-FD (II)
: 0.2 mL
CELLPACK
STROMATOLYSER-FD (I)
: 0.967 mL
Figure 10-8: Differential Sample Flow
(1) The whole blood is aspirated from the whole blood aspiration tube to the sample
rotor valve.
(2) The 33.0 µL sample (measured by the sample rotor valve) is transferred to reaction
chamber. 0.967 mL of reagent (STROMATOLYSER-FD (I)) and then 0.2 mL of
reagent (STROMATOLYSER-FD (II)) are added to make 1:36 dilution.
(3) During the approximately 8.5-second interval in the reaction chamber, the red
blood cells are hemolyzed.
(4) The sheath injector piston slowly sends 30.4 µL of 1:36 diluted sample to the
WBC-4DIFF detector; then the system, using the flow cytometry by means of
semiconductor laser, performs the analysis.
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