SYSMEX SF-3000 - Calculating the Compensation Factor; Hgb;Hct Manual

CALIBRATION
Sysmex SF-3000 Operator's Manual -- Revised September 1995 7-15
3. HGB/HCT MANUAL
HGB and HCT values can also be calibrated manually by entering the updated calibration
values using the numerical keys. The compensation factor value changed by the manual
calibration program should be within a range of 100±20%. If calibration beyond this
range is required, contact the Sysmex service representative.
3.1 Calculating the Compensation Factor
(1) Using the reference method, analyze 5 to 10 samples 3 times each to find the HGB
and HCT values.
(2) Thoroughly mix the same samples, then analyze them in the Manual mode.
(3) If there is a difference between the data measured with the SF-3000 and the
reference values measured with the reference methods, calculate new compensation
factors by using the following formula:
New Compensation Value = Current Compensation Value x
Reference mean
Instrument mean
[Example]
Reference HGB mean = 15.6 g/dL
SF-3000 HGB mean = 15.5 g/dL
Current HGB compensation factor = 100.0%
100.0 x
15.6
15.5
= 100.65 or 100.7% (rounded)
Therefore, the new compensation factor for HGB should be entered as 100.7%.
The compensation factor has increased by 0.7%.