SYSMEX SF-3000 User Manual

FUNCTIONAL DESCRIPTIONS
10-6 Sysmex SF-3000 Operator's Manual -- Revised September 1995
4.2 HGB Analysis Flow
In HGB (hemoglobin) analysis, the volume of hemoglobin contained in the blood sample
is measured. The following illustrates the procedure.
SRV
(Blood Sample : 3.0 µL)
LED
Receiver
HGB Flow Cell
SULFOLYSER : 0.5 mL
CELLPACK : 0.997 mL
Figure 10-7: Hemoglobin Sample Flow
(1) The whole blood is aspirated from the whole blood aspiration tube to the sample
rotor valve.
(2) The 3.0 µL sample (measured by the sample rotor valve) is diluted to 1:333 with
0.997 mL of diluent (CELLPACK). The diluted sample is then sent to the flow
cell.
At the same time, 0.5 mL of the reagent used for the HGB determination
(SULFOLYSER) is added to hemolyze the red cells and transform hemoglobin into
SLS-hemoglobin. The final dilution ratio is 1:500.
(3) The light emitted at a 555 nm wavelength from the photo diode passes through the
lens and is directed at the sample in the flow cell. The concentration of the SLS-
hemoglobin is measured by its absorbance of light, as compared to the absorbance
of the diluent (blank) measured before the sample entered the flow cell.

SYSMEX SF-3000 Specifications

General

Brand	SYSMEX
Model	SF-3000
Category	Measuring Instruments
Language	English