FUNCTIONAL DESCRIPTIONS
10-38 Sysmex SF-3000 Operator's Manual -- Revised March 1996
13. REAGENT SYSTEM
The reagent system consists of three types of reagents: (1) Diluent, (2) Lyse Reagents,
and (3) Detergent. Refer to Table 10-1 on the next page for further information.
Diluent: CELLPACK Used to dilute whole blood specimens to
determine hemoglobin, to count and size the
red blood cells and platelets, and determine
hematocrit. Also, this reagent forms a
laminar sheath flow around the diluted
specimen through WBC flow cell where
white blood cell counting and sizing take
place.
Lyse: STROMATOLYSER-FD (I) Used in combination with
STROMATOLYSER-FD (II) to eliminate red
blood cell stroma for accurate counting and
sizing of the Lymphocytes, Monocytes,
Eosinophils, and the group of Neutrophils
and Basophils.
STROMATOLYSER-FD (II) Used in combination with
STROMATOLYSER-FD (I) to eliminate red
blood cell stroma for accurate counting and
sizing of the Lymphocytes, Monocytes,
Eosinophils, and the group of Neutrophils
and Basophils.
STROMATOLYSER-FB Lyses red blood cells and eliminates white
blood cell stroma other than Basophils to
count and size Basophils, and to determine
WBC total count. The denucleated white
blood cells will be shrunken to be smaller
than other blood cells.
SULFOLYSER Lyses red blood cells and releases
hemoglobin for measurement by the SLS-
Hemoglobin Method.
Detergent: CELLCLEAN Strong alkaline detergent which removes lyse
reagents, cellular residuals, and blood
proteins remaining in the hydraulics.