SYSMEX CyFlow Ploidy Analyser User Manual

76 pages

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With CyView™ 1.8

Doc. No.: CY-S-3039IFUEN | Rev: 006 | Rev. date: 27-10-2021 | EN | CN 1434

CyFlow™ Ploidy Analyser

Operating Manual

Table of Contents3
Identification7
Product Information7
Manufacturer7
Introduction8
About this Operating Manual8
Typographical Conventions9
Safety Information9
Text Highlights10
General Conditions11
CE Compliance11
Sysmex Representatives12
Authorised Distributors12
Safety13
Intended Use13
Prohibited Use13
Reasonably Foreseeable Misuse13
User Definition13
User Qualification Groups14
User Qualification Matrix14
Environmental Conditions14
Prohibition and Warning Signs on the Device15
Personal Protective Equipment15
General Safety16
Laser Radiation Hazards16
Electrical Hazards16
Alterations to the Device16
Overview17
Device17
Figure 1: Front and Left Side of the Device17
Figure 2: Ports for External Devices18
Figure 3: Rear Side of the Device18
Components of the Device19
Control Elements19
Computer Interface Ports19
Sheath Fluid and Waste Bottle19
Figure 4: Sample Port at the Right Side of the Device19
Sample Port with Electrodes20
Type Plate20
Figure 5: Type Plate20
Scope of Delivery21
Scope of Operation21
Reagents22
Accessories and Spare Parts23
Transport and Storage23
Installation24
Positioning the Device24
Installation and Uninstallation24
Operation25
Switching on the Device25
Logging in26
Priming the Device26
Figure 6: Selecting Prime Mode26
Running a Quality Check27
Performing a Measurement (Sample Port)27
Saving Acquired Data28
Figure 7: Selecting Work Mode28
Figure 8: Selecting FCS-File Storage28
Performing Intermediate Cleaning29
Shutdown Procedure29
Shutdown Cleaning29
Figure 9: Intermediate Cleaning29
Switching off the Device30
Software Operation30
Fault, Cause and Remedy30
Sheath Fluid, Waste and Other Fluids30
Figure 10: Selecting Shutdown30
Calibration and Count Check Beads (Low, Medium, High)32
Measurements and Data Acquisition32
Maintenance33
Maintenance Overview33
Maintenance Procedures34
Sheath Fluid Refilling34
Inline Filter Renewal34
Other Maintenance35
Device Cleaning35
Technical Service36
Preventive Maintenance36
Defective Device36
Disposal37
Disposal of Device37
Disposal of Components37
Waste Disposal37
Sheath Fluid Disposal38
Troubleshooting40
Fault, Cause and Remedy40
Sheath Fluid, Waste and Other Fluids40
Calibration and Count Check Beads (Low, Medium, High)41
Measurements and Data Acquisition41
Maintenance42
Maintenance Overview42
Maintenance Procedures43
Sheath Fluid Refilling43
Inline Filter Renewal43
Other Maintenance44
Device Cleaning44
Technical Service45
Preventive Maintenance45
Defective Device45
Disposal46
Disposal of Device46
Disposal of Components46
Waste Disposal46
Sheath Fluid Disposal47
Software48
User Management48
Login48
User Administration48
Figure 11: Login and System Check Window48
Figure 12: User Administration Window (Exemplary)49
Overview50
Title Bar50
Drop down Menu50
Main Window51
Controls51
Figure 13: Main Window (DNA-Dapi_C.cvc85 Configuration Active)51
Figure 14: File Operations51
Data Management52
Figure 15: Device Realtime Display52
Figure 16: Acquisition Controls52
Figure 17: Intermediate Cleaning52
Open (.Fcs)53
Save (.Fcs)53
Open Configuration53
Save Configuration53
Data Acquisition Controls54
Acquisition Controls54
Device Realtime Display55
Settings56
Compensation57
Figure 18: Compensation Tab57
Plots58
Figure 19: Plots Register (Histogram)58
Figure 20: Plots Register (Dot Plot)58
Results59
Figure 21: Results Register59
Regions61
Figure 22: Creating a Quadrant Region61
Figure 23: Creating a Vertical Region61
Figure 24: Create a Polygonal Region62
Figure 25: Display Options63
Figure 26: Show Regions (Density Colored)63
Figure 27: Show Regions (Unicolored)63
Figure 28: Show All Events + Regions (Unicolored)63
Figure 29: Analysis Options64
Figure 30: Peak Analysis Result Table65
Figure 31: Region Properties66
General67
Figure 32: General Register67
Acquisition68
Figure 33: Acquisition Register68
Measure (Sample Port)69
Figure 34: Measure Register69
Figure 35: Acquisition Process for All Sample Port Measure Modes70
Figure 36: Workstep Register71
Technical Data72
Device Specifications72
Environmental Conditions74
Optical Specifications74
Fluidic Specifications75

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SYSMEX CyFlow Ploidy Analyser Specifications

General

Type	Flow Cytometer
Measurement Principle	Flow Cytometry
Excitation Wavelength	488 nm
Data Output	FCS format, CSV, PDF
Data Analysis	cell cycle analysis
Software	CyView
Applications	Ploidy analysis, cell cycle analysis
Light Source	Laser Diode

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