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CyFlow Ploidy Analyser
SYSMEX CyFlow Ploidy Analyser User Manual
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Doc. No.: CY-S-
3039
IFU
EN | Rev: 006
| Rev. date:
27
-
10
-
202
1
| EN | CN 1434
Cy
Fl
o
w™
Pl
o
id
y
Ana
l
ys
er
Op
e
ra
ti
ng
Ma
n
ua
l
2
Table of Contents
Table of Contents
3
Identification
7
Product Information
7
Manufacturer
7
Introduction
8
About this Operating Manual
8
Typographical Conventions
9
Safety Information
9
Text Highlights
10
General Conditions
11
CE Compliance
11
Sysmex Representatives
12
Authorised Distributors
12
Safety
13
Intended Use
13
Prohibited Use
13
Reasonably Foreseeable Misuse
13
User Definition
13
User Qualification Groups
14
User Qualification Matrix
14
Environmental Conditions
14
Prohibition and Warning Signs on the Device
15
Personal Protective Equipment
15
General Safety
16
Laser Radiation Hazards
16
Electrical Hazards
16
Alterations to the Device
16
Overview
17
Device
17
Figure 1: Front and Left Side of the Device
17
Figure 2: Ports for External Devices
18
Figure 3: Rear Side of the Device
18
Components of the Device
19
Control Elements
19
Computer Interface Ports
19
Sheath Fluid and Waste Bottle
19
Figure 4: Sample Port at the Right Side of the Device
19
Sample Port with Electrodes
20
Type Plate
20
Figure 5: Type Plate
20
Scope of Delivery
21
Scope of Operation
21
Reagents
22
Accessories and Spare Parts
23
Transport and Storage
23
Installation
24
Positioning the Device
24
Installation and Uninstallation
24
Operation
25
Switching on the Device
25
Logging in
26
Priming the Device
26
Figure 6: Selecting Prime Mode
26
Running a Quality Check
27
Performing a Measurement (Sample Port)
27
Saving Acquired Data
28
Figure 7: Selecting Work Mode
28
Figure 8: Selecting FCS-File Storage
28
Performing Intermediate Cleaning
29
Shutdown Procedure
29
Shutdown Cleaning
29
Figure 9: Intermediate Cleaning
29
Switching off the Device
30
Software Operation
30
Fault, Cause and Remedy
30
Sheath Fluid, Waste and Other Fluids
30
Figure 10: Selecting Shutdown
30
Calibration and Count Check Beads (Low, Medium, High)
32
Measurements and Data Acquisition
32
Maintenance
33
Maintenance Overview
33
Maintenance Procedures
34
Sheath Fluid Refilling
34
Inline Filter Renewal
34
Other Maintenance
35
Device Cleaning
35
Technical Service
36
Preventive Maintenance
36
Defective Device
36
Disposal
37
Disposal of Device
37
Disposal of Components
37
Waste Disposal
37
Sheath Fluid Disposal
38
Troubleshooting
40
Fault, Cause and Remedy
40
Sheath Fluid, Waste and Other Fluids
40
Calibration and Count Check Beads (Low, Medium, High)
41
Measurements and Data Acquisition
41
Maintenance
42
Maintenance Overview
42
Maintenance Procedures
43
Sheath Fluid Refilling
43
Inline Filter Renewal
43
Other Maintenance
44
Device Cleaning
44
Technical Service
45
Preventive Maintenance
45
Defective Device
45
Disposal
46
Disposal of Device
46
Disposal of Components
46
Waste Disposal
46
Sheath Fluid Disposal
47
Software
48
User Management
48
Login
48
User Administration
48
Figure 11: Login and System Check Window
48
Figure 12: User Administration Window (Exemplary)
49
Overview
50
Title Bar
50
Drop down Menu
50
Main Window
51
Controls
51
Figure 13: Main Window (DNA-Dapi_C.cvc85 Configuration Active)
51
Figure 14: File Operations
51
Data Management
52
Figure 15: Device Realtime Display
52
Figure 16: Acquisition Controls
52
Figure 17: Intermediate Cleaning
52
Open (.Fcs)
53
Save (.Fcs)
53
Open Configuration
53
Save Configuration
53
Data Acquisition Controls
54
Acquisition Controls
54
Device Realtime Display
55
Settings
56
Compensation
57
Figure 18: Compensation Tab
57
Plots
58
Figure 19: Plots Register (Histogram)
58
Figure 20: Plots Register (Dot Plot)
58
Results
59
Figure 21: Results Register
59
Regions
61
Figure 22: Creating a Quadrant Region
61
Figure 23: Creating a Vertical Region
61
Figure 24: Create a Polygonal Region
62
Figure 25: Display Options
63
Figure 26: Show Regions (Density Colored)
63
Figure 27: Show Regions (Unicolored)
63
Figure 28: Show All Events + Regions (Unicolored)
63
Figure 29: Analysis Options
64
Figure 30: Peak Analysis Result Table
65
Figure 31: Region Properties
66
General
67
Figure 32: General Register
67
Acquisition
68
Figure 33: Acquisition Register
68
Measure (Sample Port)
69
Figure 34: Measure Register
69
Figure 35: Acquisition Process for All Sample Port Measure Modes
70
Figure 36: Workstep Register
71
Technical Data
72
Device Specifications
72
Environmental Conditions
74
Optical Specifications
74
Fluidic Specifications
75
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SYSMEX CyFlow Ploidy Analyser Specifications
General
Brand
SYSMEX
Model
CyFlow Ploidy Analyser
Category
Measuring Instruments
Language
English
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