SYSMEX CyFlow Ploidy Analyser - Calibration and Count Check Beads (Low, Medium, High); Measurements and Data Acquisition

7 Operation
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CyFlow™ Ploidy Analyser | Operating Manual | October 2021
7.8.2 Calibration and Count Check Beads (low, medium, high)
Fault
Remedy
The result of Count Check
Beads (low, medium, high)
measurement is not within
± 10% range of the
lot-specific concentration
stated on the bottle
Shake the bottle vigorously (e.g. by vortexing) and repeat
the measurement.
Check the date of expiry of the Count Check Beads (low,
medium, high).
Perform a priming procedure.
Perform a cleaning procedure.
The result of the Calibration
Beads measurement is not
in the preselected regions of
the original configuration
script
Check within the software if you have loaded the correct
configuration file for your measurement.
Shake the bottle vigorously (e.g. by vortexing) and repeat
the measurement.
Check the date of expiry of the Calibration Beads.
Check the speed settings.
Perform a priming procedure.
Perform a cleaning procedure.
7.8.3 Measurements and data acquisition
Fault
Remedy
The device emits an
unusual / noisy sound after
starting the measurement
Check that the Waste bottle is closed properly and that
the bottle is not cracked.
Check all visible tubing and make sure that they are not
pinched.
Check that liquid (Sheath Fluid and sample) is dropping
into the Waste bottle after starting the measurement.
There is no data acquisition
visible (peaks/dots in
histogram/dot plot) during
the RUN phase
Check within the software if the laser/s is/are switched
on.
Check gain values and the threshold.
Increase the flow rate.