SAMPLE PROCESSING, Auto Mode Procedures
Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-29
4.3 Auto Mode
Auto (sampler) mode operation can be performed when the system is in the READY
mode. This mode permits automatic mixing, aspiration, and analysis of samples.
Analysis of the sample is performed in the following steps:
1. Collect and prepare sample
2. Input sample ID number
3. Analyze sample
4. Display analysis results
5. Output analysis results
6. Perform post-analysis procedures
1 . Collect and Prepare Sample
(1) Collect sample.
Draw the specified amount of blood (corresponding to the amount of EDTA
anticoagulant).
CAUTION: • Some anticoagulants will alter test results due to their effects on
hemolysis and blood platelet agglutination. Therefore, use K
2
EDTA, K
3
EDTA, or Na
2
EDTA as the anticoagulant.
• Samples must be warmed to room temperature for approximately 30
minutes before analysis.
Sample volumes required for analyses are shown below.
Table 2-5: Volume of Sample Required
Diameter of Sample Tube
12 mm 15 mm
Required sample volume
1.0 to 5.0 mL 1.0 to 7.0 mL
Aspirated sample volume
Approx. 270 µL
Use the type of blood collection tube shown below
for auto mode analyses.
Diameter a: 12 - 15 mm
Length b: 75 mm
Overall length c: 82 mm or less
a
b
c
Figure 2-19: Tube Dimensions