TROUBLESHOOTING
8-22 Sysmex SF-3000 Operator's Manual -- Revised September 1995
Sample Not Asp Error
Phenomena This error occurs during auto mode or manual closed mode analysis only.
Sufficient blood sample volume was not aspirated. (One sensor at SRV
cannot detect the sample blood.)
The sample is not processed, and data is reported by "----" as an analysis
error. The "Check Stored Data" message also appears.
If the system is set to be stopped by this error, the system will be STAT ready
for the sample analysis in manual or capillary mode.
If the system is set to continue analysis when this error occurs, the system
will process the next sample.
Probable Cause 1) Abnormal blood sample
• Clumps present in blood sample
• Extremely low concentration of blood
2) Bent, blocked or defective piercer needle, SRV and/or sampler whole
blood aspiration tubing
3) Aspiration tubing is disconnected from the sampler or the SRV.
Corrective Action 1) Check the blood sample visually, and repeat the analysis. If the blood
sample is of extremely low concentration (HGB < 3.0 g/dL), analyze the
sample in the manual mode.
2) Check and if the whole blood aspiration tube is disconnected, reconnect.
3) Check piercer, SRV and whole blood aspiration line.
Clean them as follows:
(a) Perform the shutdown sequence using CELLCLEAN (or 5% filtered
sodium hypochlorite). Refer to
Chapter 5, Section 3.1: Shutdown
.
(b) When the shutdown sequence is complete, press [Auto Rinse] key to
check the background count. When the background check is
complete and system enters Ready, reanalyze the sample.
(c) If the error persists, aliquot CELLCLEAN into 3 collection tubes and
analyze these samples in the auto mode to clean the piercer and the
whole blood aspiration line.
(d) If the error still persists, it is assumed that the piercer is blocked by
clots. Replace the piercer. Refer to
Chapter 5, Section 8.2: Replace
the Piercer
.
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