SYSMEX SF-3000 User Manual

FUNCTIONAL DESCRIPTIONS
Sysmex SF-3000 Operator's Manual -- Revised September 1995 10-5
4. RBC/PLT/HGB ANALYSIS
4.1 RBC/PLT Analysis Flow
In RBC (red blood cell) and PLT (platelet) analyses, the number of red blood cells and
platelets contained in the blood sample are counted. The following illustrates the
procedure.
SRV
Mixing
Chamber
(Blood Sample: 4.0 µL)
CELLPACK
RBC/PLT Detector
(1:500 Dilution : 40 µL)
1.96 mL 1.996 mL
Figure 10-6: RBC/PLT Sample Flow
(1) The whole blood is aspirated from the whole blood aspiration tube to the sample
rotor valve.
(2) The 4.0 µL sample (measured by the sample rotor valve) is diluted to 1:500 with
1.996 mL of diluent (CELLPACK), and then sent to the RBC sample mixing
chamber.
(3) The 40.0 µL of the 1:500 diluted sample (measured by the sample rotor valve) is
diluted to 1:25000 with 1.96 mL of diluent (CELLPACK), and then sent to the
RBC/PLT detector.
(4) The RBC detector counts RBC and PLT by means of the DC detection method. At
the same time, HCT (hematocrit) is measured by the cumulative pulse height
detection method.

SYSMEX SF-3000 Specifications

General

Brand	SYSMEX
Model	SF-3000
Category	Measuring Instruments
Language	English