SAMPLE PROCESSING, Manual Closed Mode Procedures
Sysmex SF-3000 Operator's Manual -- Revised September 1995 2-37
4.4 Manual Closed Mode
Analyses in Manual Closed mode can be performed when the system is in the READY
mode. In this mode, samples are manually mixed, set one at a time into a rack, and
analyzed.
Analysis of the sample is performed in the following steps:
1. Collect and prepare sample
2. Select closed mode
3. Input sample ID number
4. Analyze sample
5. Display analysis results
6. Output analysis results
7. Perform post-analysis procedures
1 . Collect and Prepare Sample
Draw the specified amount of blood (corresponding to the amount of EDTA
anticoagulant).
CAUTION: • Some anticoagulants will alter test results due to their effects on
hemolysis and blood platelet agglutination. Therefore, use K
2
EDTA, K
3
EDTA, or Na
2
EDTA as the anticoagulant.
• Samples must be warmed to room temperature for approximately
30 minutes before analysis.
The height of the blood sample tube should be 75 mm or shorter. Sample volumes are
shown below.
Required sample volume: 1.0 mL or more
Aspirated sample volume: Approx. 270 µL
Use the type of blood collection tube shown below
for Manual Closed analysis.
Diameter a: 12 - 15 mm
Length b: 75 mm
Overall length c: 82 mm or less
a
b
c
Figure 2-28: Tube Dimensions