INTRODUCTION
1-4 Sysmex SF-3000 Operator's Manual -- Revised September 1995
4. SYSTEM OVERVIEW
The purpose of the SF-3000 is to provide accurate results on 23 parameters and to detect
abnormalities in the sample. To assist the laboratory in the screening of abnormalities,
the SF-3000 analyzes and processes the data obtained from the analyzer unit, and the
LCD displays and outputs either a POSITIVE or a NEGATIVE message for each sample.
A NEGATIVE message signifies that parameter values for the sample are within
acceptable limits and the sample does not require any additional verification of those
values. A POSITIVE message signifies that the sample is not within the acceptable limits
and requires further review and investigation. Proper use of the instrument requires each
laboratory to establish the acceptable limits for results.
The SF-3000 uses the optical detector block incorporating the semiconductor laser to
analyze the WBC population. In the DIFF channel, lymphocytes, monocytes,
eosinophils, and the group of neutrophils and basophils are analyzed to determine the
WBC 4-differentials using flow cytometry. Basophil determination and WBC number
counting are performed in the BASO channel. RBCs and PLTs are analyzed by the RBC
detector block using the DC detection method. HGB determination is performed using
the SLS hemoglobin detection method.
The SF-3000 consists of two major systems.
• Main Unit : Houses the hydraulic and electronic systems and their control
components. The unit analyzes, processes and displays the data
on the LCD screen.
• Pneumatic Unit : Supplies the required pressure and vacuum for analysis.
Main Unit
Pneumatic Unit
Sampler Unit
(Optional)
Figure 1-1: Sysmex SF-3000 Hematology Analyzer
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