CALIBRATION
Sysmex KX-21 Operator’s Manual -- Revised October 1998 6-1
1. INTRODUCTION
Calibration is performed to compensate for any inaccuracies of the pneumatic, hydraulic, and
electric systems which will affect analysis results. This is very important in maintaining the
system accuracy.
Calibration is carried out by entering calibration values into the unit. Your Sysmex service
representative will perform initial calibration of your unit at installation. After installation,
the operator should make periodical calibration and proper quality control to maintain the
accuracy. This chapter describes calibration procedure.
1.1 Calibration Execution Timing
Calibration need not be performed in specified intervals, but when QC data varies with time,
HGB and HCT should be calibrated. When abnormal QC data occur as a result of
instrument problem, reagent degradation, or deterioration of control blood, however, do not
perform calibration.
1.2 Samples Used for Calibration
For calibration, use five or more samples of fresh normal blood that meets the following
conditions:
• Blood of a healthy person who is not taking any medicine
• Blood added with appropriate anticoagulant
• Per-sample whole blood volume to exceed 2 mL
• HGB value to exceed 10.0 g/dL
• HCT value to be within 35.5% - 55.5%
NOTE: • For calibration, do not use EIGHTCHECK-3WP, which was not
prepared for calibration but for the use as control blood.
1.3 Reference Values
Five or more normal blood samples prepared for calibration of HGB and HCT should be
accurately analyzed three times each in accordance with the reference method. The
measurements thus obtained are used as reference values.
HGB values: Cyanmethemoglobin method
HCT values: Microhematocrit method
(Five samples are used for automatic calibration.)
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