FUNCTIONAL DESCRIPTION
Sysmex KX-21 Operator’s Manual -- Revised October 1998 9-5
4. CBC ANALYSIS
4.1 WBC/HGB Analysis Flow
In WBC and HGB analysis, the volume of WBC and hemoglobin in the blood are measured.
The flow of WBC/HGB analysis is described below:
WBC Transducer
Sample Rotor Valve
Whole Blood Mode : Diluent 1.994 mL
Whole Blood 6 µL
Pre-diluted Mode : Diluent 1.922 mL
1:26 Diluted Sample 78 µL
Whole Blood Mode 1 mL
Pre-diluted Mode 1 mL
WBC/HGB Lyse:
Whole Blood Mode 1.0 mL
Pre-diluted Mode 1.0 mL
HGB Flow Cell
Tube or Microtube
Whole Blood Mode: 50 µL
Pre-diluted Mode: 1:26 Diluted Sample 200 µL
Diluent: 2.0 mL
Figure 9-4-1: WBC/HGB Analysis Flow
• Whole Blood Mode
(1) Blood is aspirated from the sample probe into the sample rotor valve.
(2) 6 µL of blood measured by the sample rotor valve is transferred to the WBC
transducer chamber along with 1.994 mL of diluent. At the same time, 1.0 mL of
WBC/HGB lyse is added to prepare 1:500 dilution sample.
When the solution is made to react in this status for approximately 10 seconds, RBC is
hemolyzed and platelets shrink, with WBC membrane held as they are. At the same
time, hemoglobin is converted into red colored methemoglobin.
(3) Of the diluted/hemolyzed sample in the WBC transducer chamber, approximately 1 mL
is transferred to the HGB flow cell.
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