FUNCTIONAL DESCRIPTION
9-6 Sysmex KX-21 Operator’s Manual -- Revised October 1998
(4) 500 µL of sample in the WBC transducer is aspirated through the aperture. The pulses
of the blood cells when passing through the aperture are counted by the DC detection
method.
(5) In the HGB flow cell, 555 nm wavelength beam irradiated from the light emitting
diode (LED) is applied to the sample in the HGB flow cell. Concentration of this
sample is measured as absorbance. This absorbance is compared with that of the
diluent alone that was measured before addition of the sample, thereby calculating
HGB (hemoglobin value).
• Pre-diluted Mode
(1) Blood sample that was diluted beforehand to 1:26 dilution using CELLPACK. This
sample is aspirated from the sample probe into the sample rotor valve.
(2) 78 µL of diluted blood measured by the sample rotor valve is transferred to the WBC
transducer chamber along with 1.922 mL of diluent. At this time, 1.0 mL of
WBC/HGB lyse is added to prepare 1:1000 dilution sample.
When the solution is made to react in this status for approximately 10 seconds, RBC is
hemolyzed and platelets shrink, with WBC membrane held as they are. At the same
time, hemoglobin is converted into red colored methemoglobin.
(3) Of the diluted/hemolyzed sample in the WBC transducer chamber, approximately 1 mL
is transferred to the HGB flow cell.
(4) 500 µL of sample in the WBC transducer chamber is aspirated through the aperture.
The pulses of the blood cells when passing through the aperture are counted by the DC
detection method.
(5) In the HGB flow cell, 555 nm wavelength beam irradiated from the light emitting
diode (LED) is applied to the sample in the HGB flow cell. Concentration of this
sample is measured as absorbance. This absorbance is compared with that of the
diluent alone that was measured before addition of the sample, thereby calculating
HGB (hemoglobin value).
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