FUNCTIONAL DESCRIPTION
Sysmex KX-21 Operator’s Manual -- Revised October 1998 9-7
4.2 RBC/PLT Analysis Flow
In RBC/PLT analysis, RBC and platelet count in the blood are measured. The flow of
RBC/PLT analysis is described below:
• Whole Blood Mode
Mixing Chamber
(1:500 Diluted Sample: 40 µL)
Tube
(Whole Blood: 50 µL)
Sample Rotor Valve
Diluent: 2.0 mL
Diluent 1.996 mL
Whole Blood 4 µL
Diluent 1.960 mL
1:500 Diluted Sample 40 µL
RBC/PLT Transducer
Figure 9-4-2: RBC/PLT Analysis Flow in Whole Blood Mode
(1) Blood is aspirated from the sample probe into the sample rotor valve.
(2) 4.0 µL of blood measured by the sample rotor valve is diluted into 1:500 with 1.996
mL of diluent and brought to the mixing chamber as diluted sample. (1st step dilution)
(3) Out of the 1:500 dilution sample, 40 µL is measured by the sample rotor valve, diluted
into 1:25000 with 1.960 mL of diluent, then transferred to the RBC/PLT transducer
chamber. (2nd step dilution)
(4) 250 µL of the sample in the RBC/PLT transducer chamber is aspirated through the
aperture. At this time, RBC and PLT are counted by the DC detection method.
At the same time, HCT (hematocrit value) is calculated by RBC pulse height detection
method.
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