SAMPLE ANALYSIS
2-16 Sysmex KX-21 Operator’s Manual -- Revised October 1998
4.2 Pre-Diluted (PD) Mode
In this mode, a sample is diluted into 1:26 before analysis. This mode is applied in analyzing
a capillary blood collected from the earlobe or fingertip. Samples are processed by the
following steps:
1. Collecting and preparing samples
2. Preparing analysis samples in PD mode (dilution of 1:26)
3. Selecting Pre-Diluted mode
4. Inputting sample No.
5. Analyzing samples
1. Collecting and preparing samples
Dilute samples to the ratio of 1:26 using CELLPACK dispensed beforehand in
containers.
[Example]
20 µL of blood is diluted in 500 µL of CELLPACK.
• A capillary blood collected from the earlobe or fingertip is prone to
platelet agglutination, so dilute and analyze it within 30 minutes.
Otherwise, correct analysis result may not be obtained.
• With a sample of blood collected from the earlobe or fingertip, blood cell
count is generally higher than normal, with lower reproducibility. If
possible, analyze the same diluted sample twice and compare the
results. When the blood collection tube containing a commercial anti-
coagulant is used, there is a possibility that RBC hemolysis or platelet
agglutination occurs depending on the types of anticoagulant, with a
consequent change in analysis result. As anti-coagulant, use EDTA-
2K, EDTA-3K, or EDTA-2Na.
Tubes up to 80 mm in height should be used. The volume of sample is as follows:
Volume of whole blood required Approx. 20 µL or more
Volume of sample aspirated Approx. 200 µL
CAUTION
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