SYSMEX KX-21 - Page 53

SAMPLE ANALYSIS
Sysmex KX-21 Operator’s Manual -- Revised October 1998 2-17
2. Preparing analysis samples in PD mode (1:26 Dilution)
(1) Clean a container such as erlenmeyer flask, beaker, etc. with CELLPACK and remove
any dirt.
(2) Using a syringe, etc., take CELLPACK into a cleaned container.
(3) Using a 500 µL transfer pipette, dispense 500 µL of CELLPACK into a micro-tube.
(4) Using a capillary tube, etc., collect 20 µL of blood and dispense it into the micro-tube.
(5) Attach the cap and mix well.
• When preparing PD-mode analysis samples, always wear rubber
gloves. After completion of work, wash hands with disinfectant. If
your hands are contaminated by blood, etc., infection of bacteria or
the like can occur.
• A 1:26 dilution sample is prone to platelet agglutination. So, analyze
the sample within 30minntes after blood dispensing and diluting. If
diluent is dispensed in advance, evaporation and dirt mixing will result
in errors in analysis values. Therefore prepare one sample at a time.
When preparing a 1:26 dilution sample, use the tools listed below:
• Diluent (CELLPACK)
• Micro-tube (MT-40, etc.)
• Capillary tube
• A 500 µL transfer pipette
• A container, such as erlenmeyer flask or beaker
• A syringe
WARNING
CAUTION