Tecnai F20 - End of Session; Imaging

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CSBCryoEMFacilityTF20OperatingInstructionsSEC
05/02/16
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IV.EndofSession
1. Closecolumnvalves
2. Retractthecampera
3. Putthescreendown
4. Home the stage (go to SearchStage and select the tab  Navigate to Control and click on                
Holder,thisensuresthatthestageisat0uminX,YandZ.
5. Removetheholdercarefully.
6. Clickoncryocycle.Makesurethecryocyclewillrunfor365minutesormore.
Wait~5minutesorforTurbototurnyellowbeforeremovingtheliquidnitrogendewar
7. Dismountthespecimenfromtheholderanddisposeofit(orsaveit)properly.
8. Once the Turbo turns yellow, remove the dewar from the microscope and dispose of the              
remainingLN2inthefoamcontainerunderthetable.
9. Placetheprotectivefabricunderneaththeanticontaminator.
10. IfusingtheRTorRTtomographyholders,returnthemtotheirproperreceptacle.
11. If using the Gatan cryo holder(s), return to the dry pumping station and follow the instructions               
thereonhowtowarmitandpumpitproperly.
12. Pleasesignupontheuser'ssheet.Recordtheactualtimespentimaging.
13. Return the next day to retrieve Data. Data will be moved overnight to the computer located               
behindthecurtainintheF20room
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V. Imaging
1. OpenDigitalMicrograph
2. Setexposuretimeto1second
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3. Wantthemeanvaluetoequal~1000
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