Tecnai F20 - Sample Loading (Cryo)

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CSBCryoEMFacilityTF20OperatingInstructionsSEC
05/02/16
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VI. SampleLoading(cryo)
1. Makesuretheanticontaminatordewarisfilledwithliquidnitrogen(LN2).
2. The cryo holder should be pumped at least 2hrs in the Dry Pumping Station (see separate               
protocol)beforeuse.Preferably,you'llhavepumpedtheholderthedaybeforeforatleast6hrs.
3. Place the cryo transfer station on the table top. Insert the cryo holder into the station and                
removetheclipringandpreviousgridifpresent.
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4. Make sure you have gathered your cryo sample in transfer dewar, forceps, large forceps for              
gridboxtransfer,andanythingelseneededandplaceitnearthetransferstation.
5. Add LN2 to both the cryo transfer station and the cryoholder. These will boil at different times.                
Do not overfill the holder nor the station, this will only cause the holder to become icy and the                  
transferstationtofrost.
6. Pluginthecontrolunitcordandturnonthetemperaturecontrolunit.
7. Tilt the compustage to 60° by going to the Search tab, flapout window, Control tab, Alpha               
toggle,typein60°andclickSetAlpha.TurntheTurboon.
8. Check that the airlock pump time is set to 6090s (Setup tab, flapout window, Settings tab,               
Defaultairlocktime:60s).
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