CSBCryoEMFacilityTF20OperatingInstructionsSEC
05/02/16
9. Cool the large forceps and transfer the grid box into the position in the transfer station. Cool the
screwdriver if needed and loosen the top on the cryo grid box and place the cover on the
transferstation.
10. Continue adding LN2 as needed. You want to keep the temperature at least 175°C or colder
(to~197°C).
11. Cool the following in the recessed bowl area: clipring tool (silver with black knobs) and attached
clipringandforcepsforthegridtransfer.
12. Open the shield on the cryo holder and transfer a grid from the grid box to the recessed grid
location on the cryo holder. Place the clip ring on (you should hear a click) and then hold the
shaft and turn the knob until both feet of the tool are together. Gently pull up with the clipring
tool.
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