Tecnai F20 - Procedure

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CSBCryoEMFacilityTF20OperatingInstructionsSEC
05/02/16
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Procedure:
During specimen insertion and retraction you should not need to use considerable force to load              
specimens. Do not force or jam anything. It could damage the compustage and/or holder. Contact              
the EM manager if you notice anything unusual (noises, smells, sounds, anything out of the ordinary               
couldsignaltrouble.Whenindoubt,pleaseask).
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1. MakesurethattheColumnValvesareclosed(SetupTab,ColumnValvesclosedisyellow).
2. Verifythattheredlightonthecompustageisoff.
3. Make sure the stage is at 0,0,0 in X, Y and Z by going to the Search Tab, Stage 2 menu, flaput                     
window,andclickonHolder.Thiswill'Home'thestage.
4. Turn on the Turbo pump on the Setup Tab (click on Turbo On, the button will go from grey to                   
orangeandwhentheturboisready,itwillbeyellow).
5. GettheRTholder,removetheplasticcoveringattheend.
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6. With the tool that's in the back part of the mounting station, place it in the small orifice in the tip                    
area(seepicture)andcarefullylifttheclamp(from3o'clockto12o'clock).
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