Tecnai F20 - Page 4

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CSBCryoEMFacilityTF20OperatingInstructionsSEC
05/02/16
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9. Fill the liquid nitrogen dewar. You'll need to fill it 2x initially. The first time a lot of LN2 will quickly                    
boil off. Wait 1015 mins after initial fill up and then top off. This should last ~4hrs. In total, for an                    
8hr work day, you'll need to fill the anticontaminator 3x. Check every 24 hours and add nitrogen                
asneeded.
10. Ifalltheseconditionsaremet,youareOKtoproceed.
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