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Teledyne ACCQPrep HP125 User Manual

Teledyne ACCQPrep HP125
142 pages
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CCQPrep HP125
Installation and Operation Guide
60-5233-812
Copyright © 2017 Teledyne Technologies Incorporated. All rights reserved.
Rev. C, January 2019

Table of Contents

Questions and Answers:

Teledyne ACCQPrep HP125 Specifications

General IconGeneral
BrandTeledyne
ModelACCQPrep HP125
CategoryLaboratory Equipment
LanguageEnglish

Summary

Safety Warnings

General Safety Precautions

General warnings and guidelines for safe operation and maintenance of the ACCQPrep HP125.

Understanding Hazard Severity Levels

Explains the different hazard levels (Caution, Warning, Danger) used in the manual.

Hazard Symbols Explained

Describes the meaning of various hazard symbols used throughout the manual.

Obtaining Additional Assistance

Provides contact information for technical support and additional information.

Section 1 Introduction

Guide Overview

Outlines the content covered in the installation and operation guide.

Product Description

Introduces the Teledyne Isco ACCQPrep HP125 chromatography system and its capabilities.

ACCQPrep System Configurations

Details available configurations including base, ELSD, and Mass Spectrometer options.

Operating Overview and Control

Explains system control via touch screen and network connections.

Safety Components and Disconnects

Identifies the power cord and circuit breaker as safety disconnects.

Technical Specifications

Lists detailed technical specifications of the ACCQPrep HP125 system.

Section 2 Installation

ACCQPrep Installation Overview

General overview of the ACCQPrep installation process.

Required Tools for Installation

Lists the necessary tools for setting up the ACCQPrep system.

Setting Up the ACCQPrep System

Instructions for initial system setup, including lifting precautions.

Pump Installation Procedure

Step-by-step guide for installing the pump assembly into the system.

Solvent Line Installation

Instructions for connecting the solvent inlet and outlet lines for the pumps.

Fluid Line Installation Steps

Details the process of installing the fluid lines for solvent delivery.

ACCQPrep with Optional ELSD Connections

Specific connections required when the ACCQPrep is equipped with the optional ELSD detector.

ELSD Carrier Gas and P-trap Setup

Instructions for connecting the carrier gas and preparing the P-trap for ELSD operation.

System Priming Steps

Guides the user through the process of priming the system with solvents.

Manual Injection Port Installation

Instructions on how to install the luer injection port for manual sample injection.

AutoSampler Installation Overview

General steps for installing the AutoSampler module onto the ACCQPrep.

Mounting the AutoSampler Z-Drive

Details on attaching the Z-Drive component to the AutoSampler's Y-axis carriage.

AutoSampler Wash Well Setup

Instructions for mounting and connecting the wash well for the AutoSampler.

AutoSampler Tubing Connections

Explains the connections for wash and waste tubing on the AutoSampler.

AutoSampler Module to ACCQPrep Tubing

Diagram and steps for connecting the AutoSampler module to the ACCQPrep via tubing.

AutoSampler and ACCQPrep USB Connections

Wiring diagram and steps for connecting the AutoSampler to the ACCQPrep using USB cables.

Establishing AutoSampler Fluid Connections

Detailed steps for making the necessary fluid connections for the AutoSampler probe.

AutoSampler Test Tube and Wash Well Placement

Placement of the test tube and wash well on the AutoSampler platform.

Powering On the AutoSampler and ACCQPrep

Instructions for powering on both the AutoSampler and the main ACCQPrep unit.

Priming the AutoSampler Wash Station

Procedure for priming the wash station of the AutoSampler.

Column Installation Overview

General information about installing HPLC columns with the ACCQPrep.

Column Mounting Procedures

Steps for mounting different diameter HPLC columns onto the ACCQPrep.

Column Mounting Parts and Tracks

Details on the components and tracks used for mounting columns.

Connecting Column Inlet and Outlet Lines

Instructions for connecting the tubing to the column inlet and outlet.

Column Configuration in Software

How to configure installed columns within the ACCQPrep software.

Optional Column Select Valve (CSVM)

Description of the CSVM accessory and its functionality for column selection.

Installation Troubleshooting

Common installation issues and their solutions.

AutoSampler Control Troubleshooting

Steps to resolve issues where AutoSampler control is not visible in the software.

Section 3 Configuration

ACCQPrep Instrument Configuration

How to configure system settings like solvents, solvent level sensing, and waste level sensing.

System Naming and Time Settings

Configuring system name, time zones, and default tube volume.

Network Configuration

Steps for connecting the ACCQPrep to a network for remote control and data saving.

User Preferences and Settings

Customizing user interface language, print reports, and pressure display.

Absorbance and Peak Hold Settings

Options for showing absorbance during equilibration and enabling automatic peak hold.

Prep HPLC Method Setup

Configuration options for preparative HPLC methods, including loop volume and probe type.

Defining a HPLC Column

Step-by-step guide to define new columns and their associated default methods.

Deleting a Defined Column

Procedure for removing a previously defined column from the system.

Creating Custom HPLC Methods

Instructions on how to create new methods associated with specific columns.

Creating Focused Gradient Methods

Guidance on developing focused gradients for optimized separations.

Section 4 Operation

Introduction to System Operation

Overview of the ACCQPrep's operational capabilities and method flexibility.

Method Selection Procedures

Explains various ways to select and edit methods for a separation.

Starting a Separation

Steps to initiate a separation, including options for ELSD, PurIon, and manual injection.

Separation with AutoInjector

Detailed steps for performing separations using the AutoInjector module.

Selecting Multiple Injections

How to set up and perform multiple injections using the AutoInjector.

AutoInjector Sample Volume and Injection Count

Setting sample volume and the number of injections for automated sequences.

AutoInjection Operating Steps

Detailed sequence of operations performed by the AutoInjector during sample injection.

Separation Using AutoSampler Module

Procedures for setting up and running separations with the AutoSampler module.

Using the Queue Tab for AutoSampler

How to program and manage sample queues for automated separations.

Queue Tab Column Explanations

Explains the various columns and their functions within the AutoSampler Queue tab.

AutoSampler Injection Techniques

Describes the default and bracketed sample injection protocols for the AutoSampler.

Default Injection Sequence Steps

Detailed step-by-step process for the default injection sequence with the AutoSampler.

Bracketed Sample Injection Sequence Steps

Detailed step-by-step process for bracketed sample injections with the AutoSampler.

Fraction Collection with AutoSampler

How the AutoSampler enhances fraction collection capabilities by extending rack usage.

Operation Troubleshooting

General troubleshooting guide for operational issues encountered during use.

Injection Valve Leak Troubleshooting

Specific steps to diagnose and resolve leaks from the injection valve.

Retention Time and Flow Rate Issues

Troubleshooting unexpected or variable retention times due to temperature or flow rate issues.

Chromatogram Peak Issues Troubleshooting

Addresses issues like no peaks, early/late elution, poor peak shape, and phantom peaks.

Baseline and ELSD Detection Issues

Troubleshooting drifting baselines and problems with ELSD detector performance.

PurIon Detection and Pressure Issues

Troubleshooting PurIon detection problems and excessive system pressure.

Troubleshooting Incorrect Fraction Volumes

Diagnosing and resolving issues related to incorrect fraction volumes collected.

Injection Valve Dripping Troubleshooting

Steps to troubleshoot and resolve dripping from the injection valve.

Section 5 PeakTrak

PeakTrak Overview and Window Elements

Introduction to the PeakTrak software and its main window components.

Understanding Method and Run Files

Explains the purpose and usage of method files and run files in PeakTrak.

PeakTrak File Menu Operations

Details the commands available in the PeakTrak File menu for managing files.

File Menu Operations Continued

Further explanation of file management operations within the PeakTrak software.

PeakTrak MS Menu Options

Describes the options available in the PeakTrak MS menu for PurIon systems.

PeakTrak Tools Menu Options

Details the functions accessible through the PeakTrak Tools menu.

PeakTrak Help Menu Options

Information on accessing help resources and software updates within PeakTrak.

PeakTrak Window Descriptions

Detailed descriptions of various windows used within the PeakTrak software.

Main Window Functionality

Explains the controls and display areas of the PeakTrak Main window.

PeakTrak MS and UV Controls

How to use the MS and UV controls within the PeakTrak interface.

PeakTrak Run Settings

Configuration options for columns, sample names, flow rates, and solvents.

PeakTrak Run Control Buttons

Explanation of the function of control buttons like PLAY, PAUSE, and STOP.

Peak Collection Control Buttons

Options for controlling how fractions are collected (ALL, PEAKS, NONE).

Viewing Peak Collection Data

How to view and interpret data related to fraction collection after a run.

PeakTrak Method Editor Interface

Description of the Method Editor window and its sections for modifying method settings.

Gradient Plot Area Controls

How to use the controls in the Gradient Plot Area to edit gradient curves.

Peak Collection Modes and Tube Volume

Setting peak collection modes and specifying the volume collected per tube.

Peak Detection Options Configuration

Enabling and configuring various peak detection methods like wavelength and slope-based.

Peak Detection Settings Details

Further details on peak detection settings such as peak width and threshold.

ELS Detector Settings and Optimization

Adjusting ELS detector parameters like spray chamber and drift tube temperatures.

Viewing Column Data

Accessing information about the installed ACCQPrep columns.

PeakTrak File Management Window

Overview of the Files window for browsing and managing files and folders.

File and Folder Operations

Performing actions like copy, paste, delete, and create new folders for files.

Setting Data Path for User Files

Configuring the default directory for saving and opening user files.

MS Method Development for PurIon

Developing and verifying ionization conditions for PurIon mass spectrometer.

PurIon MS Manual Control and Formatting

Controlling the PurIon MS manually and formatting the mass spectrum display.

PurIon Ionization Settings Adjustment

Adjusting ionization parameters to improve detection of specific compounds.

Ionization Settings Details

Advanced settings for ionization, probe selection, gas temperature, and voltage.

Manual Control Window Operations

Using the Manual Control window to pump solvents and monitor lamp energy.

Network and IP Address Configuration

Configuring network settings, IP addresses, and network printing.

Setting Administrator Password

Procedure for setting or changing the system's administrator password.

Examples of PeakTrak Actions

Demonstrates common tasks performed within PeakTrak, like editing methods.

Editing an HPLC Method

Detailed steps for modifying basic and advanced method settings to optimize separations.

Initial Waste and Time Window Settings

Configuring initial waste diversion and time windows for fraction collection.

Peak Collection Mode Selection

Choosing between All, Peaks, or None modes for collecting fractions.

Peak Detection Options and Configuration

Enabling and configuring various peak detection methods like wavelength and slope-based.

ELS Detector Settings and Optimization

Adjusting ELS detector parameters like spray chamber and drift tube temperatures.

Alternative Method File Creation

Methods for creating method files by importing or extracting from previous runs.

Defining HPLC Gradient Curves

Step-by-step guide to create and modify gradient profiles for separations.

Real-time Gradient Editing During Run

Modifying gradient profiles dynamically while a separation is in progress.

Bypassing the Injection Loop

Using the loop bypass feature for stacking injections in isocratic separations.

Monitoring Compound Purity Measure

Using wavelength ratios to monitor compound purity during elution.

Viewing Previous Run Data

Procedures for opening and reviewing chromatograms and data from past runs.

Viewing Run Data and Options

Accessing run summaries, printing, and saving run data from the viewer.

Reading and Interpreting Chromatograms

Understanding the components of the chromatogram plot, including traces and axes.

Manual Control of ACCQPrep System

Using manual control for solvent pumping and monitoring raw lamp energy.

Section 6 Maintenance

Introduction to Maintenance Routines

Overview of common maintenance tasks for the ACCQPrep HP125.

Exterior Surface Cleaning

How to clean the exterior surfaces of the instrument safely.

Collection Rack and Tray Cleaning

Procedures for cleaning conductive plastic racks and trays to prevent static discharge.

System Standby and Shutdown Procedures

Steps for placing the system in standby mode and shutting it down properly.

Monthly Tubing Inspection

Performing monthly checks on solvent, waste, and drain tubing for damage or leaks.

Preventive Maintenance Schedule

Outlines a schedule for preventive maintenance tasks based on frequency (As Needed, Every Run, Monthly, Annually).

Flow Cell Cleaning Procedures

Explains the importance and methods for cleaning the flow cell.

Post-Separation Flow Cell Flush

The default procedure of flushing the flow cell with solvent B after each separation.

Quick Flow Cell Cleaning

Steps for performing a quick cleaning of the flow cell when recommended by the system.

Monthly Flow Cell Cleaning Procedure

Detailed steps for monthly cleaning of the flow cell, often used when quick cleaning is insufficient.

ACCQPrep HP125 Component Maintenance

Overview of maintenance procedures for specific ACCQPrep components.

Injection Valve Rotor Replacement

Step-by-step guide for replacing the injection valve rotor.

Removing the Injection Valve Rotor

Detailed instructions on how to remove the injection valve rotor.

Installing a New Injection Valve Rotor

Steps for correctly installing a new injection valve rotor.

Pump Seal Replacement Procedure

Comprehensive guide for replacing the pump seals in the ACCQPrep.

Removing Pump Head and Spacer Block

Instructions for safely removing the pump head and spacer block during seal replacement.

Removing and Cleaning Pump Seals

Steps for removing old seals and cleaning the piston as part of pump seal replacement.

Replacing Pump Seals and Spacer Block

Procedures for installing new pump seals and reassembling the spacer block.

Reinstalling Pump Head and Fasteners

Final steps for reassembling the pump head and securing it with fasteners.

Seal Conditioning Process

How to condition new pump seals prior to normal operation.

Check Valve Replacement Procedure

Step-by-step instructions for removing and replacing pump check valves.

Pump Assembly Removal for Check Valve Access

Steps to remove the pump assembly to access check valves for replacement.

Disconnecting Lines and Fittings for Pump Access

Details on disconnecting various lines and fittings to facilitate pump assembly removal.

Removing and Installing Check Valve Housings

Procedures for removing old check valve housings and installing new ones.

AutoSampler Maintenance Overview

Introduction to maintenance tasks for the AutoSampler module.

Peristaltic Tubing Replacement

Step-by-step guide for replacing the peristaltic tubing on the AutoSampler.

ELSD Detector Maintenance

Overview of maintenance procedures for the ELSD detector.

Cleaning the ELSD Detector Spray Chamber

Instructions for cleaning the spray chamber of the ELSD detector.

ELSD Detector Cleaning Steps

Detailed steps for cleaning the ELSD detector's spray chamber, including solvent usage.

ELSD Detector Rinse and Temperature Adjustment

Final rinse steps and temperature adjustments for the ELSD detector after cleaning.

PurIon Maintenance Overview

Introduction to maintenance tasks specific to the PurIon mass spectrometer.

ESI and APCI Ion Source Removal

Steps to remove the ESI/APCI ion source housing from the PurIon system.

ESI and APCI Ion Source Replacement

Instructions for reinstalling the ESI/APCI ion source housing onto the PurIon.

Cleaning the Ionization Source Capillary

Procedure for cleaning the capillary inlet to the PurIon vacuum region.

Overpressure Error Troubleshooting (PurIon)

Troubleshooting steps for overpressure errors encountered with the PurIon system.

Check Valve Cleaning for PurIon

How to clean check valves in the PurIon system if they become stuck.

Replacing PurIon Check Valves

Steps for installing new check valve cartridges into the PurIon pump head.

PurIon Cone Cleaning

Procedure for cleaning the PurIon cone to resolve vacuum reading issues.

Capillary Inlet Cone Removal

Steps to remove the capillary inlet cone from the PurIon system.

Capillary Inlet Cone Cleaning

How to clean the capillary inlet cone to resolve blockages.

Capillary Inlet Cone Installation

Steps for reinstalling the capillary inlet cone onto the PurIon system.

PurIon Troubleshooting Guide

General troubleshooting for PurIon system errors and issues.

Common PurIon Error Codes and Resolutions

Table listing common PurIon error codes and their corresponding resolutions.

Appendix A

Diagrams for AutoSampler and ACCQPrep Connections

Introduces diagrams showing tubing and USB connections between the AutoSampler and ACCQPrep.

AutoSampler to ACCQPrep Tubing Diagram

Visual representation of tubing connections between the AutoSampler and ACCQPrep.

AutoSampler to ACCQPrep USB Wiring Diagram

Visual representation of USB wiring connections between the AutoSampler and ACCQPrep.

Compliance Statements

CE Declaration of Conformity

Official declaration that the equipment conforms to relevant EU directives and standards.

Teledyne ISCO One Year Limited Factory Service Warranty*

Warranty Coverage and Exclusions

Details the terms, conditions, coverage, and exclusions of the factory service warranty.

Obtaining Warranty Service and Repair

Instructions on how to obtain warranty service, contact information, and shipping guidelines.

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