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CombiFlash Rf
Teledyne CombiFlash Rf User Manual
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i
Com
bi
Flas
h
®
Rf
+
Us
e
r
M
a
n
u
a
l
F
or indoor use only
COPYRIGHT © 2013 by
Te
l
e
d
y
n
e
I
s
c
o
4700 Superior St.
Lincoln, Nebraska, U
.S
.A. 68504
Phone: (402) 464-0231
T
oll Free: (800) 228-4373
F
AX: (402) 465-3022
Revision C
, May 2015
P
art #69-5233-650
2
Table of Contents
Default Chapter
3
Table of Contents
3
1 Introduction
9
Documentation Overview
9
Product Overview
9
Operating Overview
16
Multiple Control Possibilities
16
File Storage
16
Specifications
17
Controls, Indicators, and Features of the Combiflash Rf
26
Safety
33
Hazard Severity Levels
34
Hazard Symbols
34
For Additional Information
36
2 Preparation
37
Rf + , Rf + Lumen, Rf+ Purion, and EZ Prep Systems
37
Unpacking the Unit
37
Instrument Location
39
Connect Power
39
Connect Solvent Lines (Excludes EZ Prep)
40
Connect Waste Lines (Excludes EZ Prep)
42
EZ Prep Plumbing Connections
45
External Gas
46
Combiflash Rf , Rf Lumen, and EZ Prep
46
Combiflash Rf
47
Lumen or EZ Prep with ELSD
47
Connect and Route Drain Lines
47
Only
47
Position the System
50
Install Collection Tube Racks
51
External Detector (Optional)
53
Turn on Power
54
Configure the System
54
Solvents
54
Enable Automatic Phase Change (EZ Prep Only)
55
General Settings
56
Set Date/Time
56
Vapor Limit
56
Set Default Tube Volumes
57
User Preferences
57
Prep HPLC Tab
60
Restart the System
61
Prime the Solvent Lines
62
System Verification
63
Installation Qualification Checklist
64
3 Preparation: Rf 150 Systems
66
Unpacking the Unit
66
Instrument Location
68
Connect Power
68
Connect Solvent Lines
69
Connect Waste Lines
71
Connect and Route Drain Lines
72
Position the System
74
Install Collection Tube Racks
75
Turn on Power
77
Configure the System
77
Solvents
78
General Settings
78
Set Date/Time
78
Vapor Limit (Optional)
78
Set Default Tube Volumes
79
User Preferences
79
Restart the System
82
Prime the Solvent Lines
83
System Verification
83
Installation Qualification Checklist
84
4 Preparation: Optional 4X Module
86
Unpacking the Unit
86
Instrument Location
87
Connect Power
87
Connect Solvent Lines
88
Connecting the Combiflash Rf with 4X Module
88
And Purion
89
Connect Waste Lines
90
Control Connection
98
Position the System
98
Remove Shipping Hardware
98
Turn on Power
99
Prime the Solvent Lines
100
System Verification
101
Installation Qualification Checklist
102
5 Operation
104
Flash Sample Preparation
104
Liquid Sample Injection
104
Solid Samples
104
Pre-Loading on Column
108
Loading a Redisep Rf Column
108
Start a Default Method
109
During the Run
113
Run Control Buttons
113
Bypassing the Solid Load Cartridge
116
About Solvent Level Detection
117
EZ Prep Operation
119
Column Mounting
119
Sample Preparation
120
Starting the Separation
121
6 User Help Reference
122
Peaktrak Window Elements
122
Method File
123
Run File
124
How to Get Peaktrak Help
124
File
124
Method Editor
128
Tools
129
Help
131
Main Window
132
Method Editor
138
Gradient Optimizer
146
Column Data
146
Batch
147
Files
148
Set Data Path
149
MS Method Development
150
MS Manual Control
152
Ionization Settings
152
Auto Prime/Valve Wash
155
Manual Control
155
Configuration
157
Set Administrator Password
162
User Management
162
Calibrate Touchscreen
163
Starting a Run Using Default Settings
164
Editing a Method
167
Alternative Ways to Create Method Files
173
Editing a Default Method
174
Defining a Gradient
175
Real-Time Gradient Editing
175
Bypassing the Solid Load Cartridge
176
Bypassing the Injection Loop
177
Monitoring the Purity Measure
178
Viewing Runs
179
Manual Control of the Combiflash Rf
182
Setting the Vapor Limit
183
7 Remote Interfaces
186
Network Configuration
186
Network PC Access
187
Network Printing
188
Network File Save Configuration
188
Apple Mobile Digital Devices
189
Install and Configure the Peaktrak App
189
Direct Connection
189
Windows 7 Settings
190
Windows Vista Settings
192
Windows XP Settings
194
Windows 2000 Settings
196
Completing the Direct Connection
198
Direct Printing
198
8 Maintenance
200
System Standby and Shut down
200
Preventive Maintenance
201
Cleaning
201
Collection Rack and Tray Cleaning
203
Tubing Inspection
203
Flow Cell Cleaning
205
Post Separation
205
Quick Cleaning When Recommended
206
Monthly Flow Cell Cleaning
210
Purion Maintenance
210
Removing ESI Probe
210
ESI Probe Replacement
211
APCI Probe Removal
212
APCI Probe Replacement
213
Removal of Ion Source Housing
213
ESI and APCI Replacement from Purion S and Purion L
214
Cleaning the Ionization Source Capillary
214
Replacement of Ion Source Housing
215
Overpressure Error
215
Check Valve Cleaning
216
Replacing Check Valves
217
Injection Valve Maintenance
218
Electrical Troubleshooting
218
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Teledyne CombiFlash Rf Specifications
General
Brand
Teledyne
Model
CombiFlash Rf
Category
Laboratory Equipment
Language
English
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