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CombiFlash NextGen
Teledyne CombiFlash NextGen User Manual
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Combi
Fla
sh
®
Ne
xt
Gen
Installation and Oper
ation Guide
Part Number 69-5253-032
© 2020 Teledyne Technologies Incorporated. All rights reserved.
Rev. F, December 2023
Software version 5.0.89
Use and Disclosure of Data: Informatio
n contained herein is classified as EAR99 under the U.S. Ex
port Administration Regulations.
Export, reexport or diversion contrary to
U.S. law is prohibited.
2
Table of Contents
Table of Contents
7
Section 1 Introduction
11
Overview
11
Product Overview
11
Operating Overview
16
System Control Possibilities
16
Data Storage
16
Specification
16
Controls, Indicators, and Features of Combiflash Nextgen Systems
19
Safety
23
Hazard Severity Levels
24
Hazard Symbols
24
Additional Information
25
System Pressure Management
25
Pressure Limits
26
Overpressure Conditions
27
Section 2 Preparation
29
Unpacking the System
29
Instrument Location
31
Connect Power
31
Plumbing Connections
31
External Gas
33
Combiflash Nextgen Equipped with Optional ELSD
33
Connect and Route Drain Lines
34
Vapor Enclosure (Optional)
38
Install Solid Load Cartridge Cap Ring Support and SLCC Storage Bracket (Optional)
39
Position the System
40
Install Collection Tube Racks
41
Powering on the System
43
Configure the System
43
Solvents
44
General Settings
44
Vapor Limit (if Equipped)
45
Set Default Tube Volumes
47
User Preferences
47
User Management
49
Restart the System
50
Prime the Solvent Lines
50
System Verification
53
Installation Qualification Checklist
54
Section 3 Operation
55
Flash Sample Preparation
55
Liquid Sample Injection
55
Solid Samples
55
Loading a Redisep Column
56
Starting a Separation
56
During the Separation
60
Run Control Buttons
60
Bypassing the Solid Load Cartridge (Injection Valve Equipped Systems Only)
61
About Solvent Level Detection (Systems Equipped with this Option Only)
61
Software Reference
62
Main Separation Screen
63
Method Editor Screen
65
The Method Editor Button Bar
65
Method Editor Run Settings
66
Edit Gradient Options
67
Peak Collection
67
Tube Volume
68
Peak Detection Options
68
Manual Control
73
Results Screen
74
Purion Operation
75
The Focus Gradient Generator
77
Focused Gradients for Flash Columns
78
Configuring a Column to Use a Scouting Gradient
78
Generating a Focused Gradient Method
80
Focused Gradients from Analytical HPLC
81
Configuring a Column to Use a Scouting Gradient
81
Calculating the Focused Gradients
82
Section 4 Network Configuration
85
Network Configurations
85
Network PC Access
86
Network Printing
86
Network File Save Configuration
86
Direct Connection
86
Section 5 Maintenance
89
System Standby and Shut down
89
Preventive Maintenance
89
Cleaning
90
Collection Rack and Tray Cleaning
90
Inspection
91
Tubing Inspection
91
Flow Cell Cleaning
92
Post Separation
92
Quick Cleaning When Recommended
93
Monthly Flow Cell Cleaning
95
ELSD Maintenance
95
Cleaning the ELSD Detector
95
Purion Maintenance
98
ESI and APCI Removal from Purion S and Purion L
98
ESI and APCI Replacement Purion S and Purion L
99
Cleaning the Ionization Source Capillary
99
Replacement of Ion Source Housing
99
Overpressure Error
99
Check Valve Cleaning
100
Replacing Check Valves
101
5
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Teledyne CombiFlash NextGen Specifications
General
Brand
Teledyne
Model
CombiFlash NextGen
Category
Laboratory Equipment
Language
English
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