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Teledyne CombiFlash NextGen 100 User Manual

Teledyne CombiFlash NextGen 100
104 pages
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CombiFlash
®
NextGen
Installation and Operation Guide
Part Number 69-5253-032
© 2020 Teledyne Technologies Incorporated. All rights reserved.
Rev. F, December 2023
Software version 5.0.89
Use and Disclosure of Data: Information contained herein is classified as EAR99 under the U.S. Export Administration Regulations.
Export, reexport or diversion contrary to U.S. law is prohibited.

Table of Contents

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Teledyne CombiFlash NextGen 100 Specifications

General IconGeneral
Maximum Flow Rate100 mL/min
Flow Rate Range1-100 mL/min
Detector TypeUV-Vis
Number of Detectors1
Solvent Channels4
Fraction CollectionYes
Number of Channels4
Sample Capacityup to 100 g
Power Requirements100-240 VAC, 50/60 Hz
WeightApprox. 100 lbs
TypeAutomated Flash Chromatography System
Column Size Compatibility4-100 g sample loading

Summary

CombiFlash NextGen Systems - Section 1 Introduction

1.5 Controls, Indicators, and Features

Illustrates and describes the various controls and features of the CombiFlash NextGen unit.

1.6 Safety

Provides essential safety information and general warnings for operating the CombiFlash NextGen system.

CombiFlash NextGen Systems - Section 2 Preparation

2.1 Unpacking the System

Provides instructions for carefully unpacking the CombiFlash NextGen system and inspecting contents.

2.3 Connect Power

Details the steps for connecting the CombiFlash NextGen system to the mains power supply.

2.4 Plumbing Connections

Guides on making essential plumbing connections for the CombiFlash NextGen system.

2.6 Connect and Route Drain Lines

Provides instructions for connecting and routing the drain lines for the CombiFlash NextGen system.

2.11 Powering on the System

Details the procedure for powering on the CombiFlash NextGen system and initiating startup.

2.12 Configure the System

Guides on configuring the CombiFlash NextGen system for optimal performance and user needs.

2.14 Prime the Solvent Lines

Provides instructions for priming the solvent lines of the CombiFlash NextGen system before first use.

2.15 System Verification

Recommends verifying system operation using the CombiFlash Universal verification kit.

CombiFlash NextGen Systems - Section 3 Operation

3.1 Flash Sample Preparation

Discusses methods for preparing samples, including liquid injection and solid sample cartridges.

3.2 Loading a RediSep Column

Provides step-by-step instructions for loading a RediSep column into the CombiFlash NextGen.

3.3 Starting a Separation

Guides on how to start a separation run after system installation and sample preparation.

3.5.2 Method Editor Screen

Details the Method Editor screen for controlling less common parameters not on the main screen.

3.5.11 PurIon Operation

Covers the operation, standby, and shutdown procedures for the PurIon Mass Detector.

3.7 Focused Gradients for Flash Columns

Guides on creating efficient preparative gradient methods using flash columns.

Generating a Focused Gradient Method

Details the process of generating a focused gradient method based on a scouting run.

3.8 Focused Gradients from Analytical HPLC

Guides on creating preparative gradient methods using analytical HPLC data.

Calculating the Focused Gradients

Details how to calculate focused gradients from analytical HPLC data.

CombiFlash NextGen Systems - Section 5 Maintenance

5.2 Preventive Maintenance

Outlines the minimum periodic maintenance requirements for safe and reliable operation.

5.3 Cleaning

Provides general instructions for cleaning the exterior surfaces of the CombiFlash NextGen system.

5.4 Inspection

Details routine inspection tasks for the CombiFlash NextGen system components.

5.5 Flow Cell Cleaning

Provides procedures for cleaning the flow cell to maintain performance and lamp energy.

5.5.2 Quick Cleaning when Recommended

Details the quick cleaning procedure for the flow cell when recommended by a system alert.

5.6 ELSD Maintenance

Provides guidance on periodic cleaning of the ELSD spray chamber for maximum performance.

5.7 PurIon Maintenance

Covers maintenance procedures for the PurIon Mass Spectrometer, including component removal.

5.7.1 ESI and APCI Removal from PurIon S and PurIon L

Guides on removing the ESI or APCI ion source housing from the PurIon system.

5.7.2 ESI and APCI Replacement PurIon S and PurIon L

Details the procedure for replacing the ESI or APCI ion source housing on the PurIon.

5.7.12 PurIon Troubleshooting

Offers troubleshooting steps for common PurIon instrument issues and errors.

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