Thermo Scientific Cryotome User Manual

77210163GB Issue 3
STANDARD AND ELECTRONIC
OPERATOR
GUIDE
77210163
Standard & E
ENGLISH
Issue 3
Cryotome
©

Thermo Scientific Cryotome Specifications

General

Brand	Thermo Scientific
Model	Cryotome
Category	Laboratory Equipment
Language	English

Summary

SYMBOLS

Safety Symbols and Conventions

Details general symbols, warnings, and conventions used in the manual and on the instrument.

CE Mark Compliance

Details the instrument's compliance with CE Mark requirements and relevant directives.

WELCOME

Introduction to Cryotome

Introduces the Cryotome series, its purpose, and intended users in pathology laboratories.

Essential Safety Precautions

Details important safety information, warnings, and precautions for operating the instrument.

DESCRIPTION

Cryotome Overview and Components

Describes the instrument's components, design, and key factors for good sectioning.

CONTROLS

Main Control Panel Overview

Overview of the main control panel interface, its sections, and button confirmation.

Micron Selector Operation

Details the function of the micron selector for setting section thickness.

Specimen Travel and Time Controls

Specimen Travel Indicator

Explains the specimen travel bar graph indicating available travel position.

Time Controls Functionality

Describes setting clock time, defrost times, and fumigate times.

Setting Instrument Clock and Defrost Times

Setting the Instrument Clock

Step-by-step guide to setting the instrument's clock for day, hours, and minutes.

Setting Defrost Times

Instructions for programming defrost cycles including day, hours, and minutes.

Setting Fumigate Times and Chamber Controls

Setting Fumigate Times

Instructions for programming fumigation cycles similar to defrost times.

Chamber Controls Overview

Overview of the chamber control functions like defrost, fumigate, lamp, and boost.

Immediate Fumigate Function

Immediate Fumigate Operation

Explains the immediate fumigation operation sequence and safety warnings.

Chamber Control Functions

Cancel Defrost/Fumigate, Lamp, Boost, and Lock

Details functions for cancelling cycles, operating the lamp, cryobar boost, and window lock.

Temperature and Keyswitch Controls

Temperature Controls

How to set and monitor working and cryobar temperatures.

Keyswitch Control Operation

Operation of the panel keyswitch for locking and unlocking the control panel.

Advance Control Panel

Controls for Standard and E Models

Describes rotary knobs (Standard) and buttons (E) for section thickness and specimen movement.

Message Codes and Indicators

Display Message Codes

Explains status and error messages shown on the instrument's displays.

Connections and Power Inlet

Instrument Connections

Describes power inlet, circuit breaker, and window boost switch connections.

INSTALLATION AND SETTING UP

Introduction and Unpacking

Overview of unpacking, instrument dimensions, and initial setup.

Removing Transit Fixings

Essential steps to remove internal transit restraints before operating the instrument.

Installing the Cryotome and Transit Plates

Installing the Cryotome

Instructions for positioning the instrument and providing ventilation clearance.

Microtome Transit Fixings

Details on removing and refitting microtome-specific transit fixings.

Electrical Requirements

Power Supply and Wiring

Details power supply voltages, cable types, and electrical safety requirements.

Switching the Instrument On

Procedure for Powering On

Step-by-step procedure for switching the instrument on and initial checks.

Switching Off and Preparing for Use

Procedure for Powering Off

Procedure for safely switching the instrument off.

Preparing the Instrument for Use

General steps before operating the instrument, including safety and locking.

Fitting Knife Holders and Standard Knives

Fitting a Knife Holder

Instructions for securely installing different types of knife holders.

Fitting a Standard Solid Knife

Steps to safely install a standard solid knife into the holder.

Setting Up Knife Holder and Anti-Roll Plate (Standard Knives)

Standard Knife Setup

Adjusting the knife holder angle and anti-roll plate for standard knives.

Fitting a Disposable Blade

Fitting Disposable Blades

Instructions for safely installing disposable blades using the blade transporter.

Specimen Head and Orientation Heads

Setting Up for Disposable Blades

Setting up the knife holder and anti-roll plate for disposable blades.

Specimen Head and Orientation Heads

Describes the specimen head, cryocassette clamp, and orientation heads.

Fixed and Fine Orientation Heads

Fixed Orientation Head Details

Details for securing the cryocassette in the fixed orientation head.

Fine Orientation Head Details

Details for adjusting specimen orientation using the fine orientation head.

Coarse Orientation Head and Changing Heads

Coarse Orientation Head Operation

Details for adjusting specimen orientation using the coarse orientation head.

Procedure for Changing Orientation Heads

Procedure for removing and replacing the different orientation heads.

OPERATION

Introduction and Mounting a Specimen

Overview of instrument operation and step-by-step guide for mounting a specimen.

Specimen Preparation and Freezing

Setting Temperature and Embedding

Details on setting chamber temperature and embedding specimens for freezing.

Setting Knife Holder for Cutting

Knife Holder Adjustment for Cutting

Adjusting the knife holder and specimen proximity for optimal sectioning.

Cutting Sections

Procedure for Section Cutting

Procedure for cutting tissue sections using the handwheel and micron selector.

Removing Specimen and Housekeeping

Removing Specimen from Cryocassette

How to remove the cut specimen from the cryocassette after sectioning.

Daily Housekeeping Tasks

Daily cleaning and maintenance tasks to ensure instrument hygiene.

CLEANING AND MAINTENANCE

General Cleaning and Maintenance Guidelines

General advice on instrument cleaning, decontamination, and maintenance.

Fumigation Procedure

Instrument Fumigation Steps

Steps for performing instrument fumigation using formalin solution.

Instrument Cleaning

Cleaning the Refrigerated Chamber

Detailed cleaning instructions for the refrigerated chamber and its components.

Knife Holder Cleaning and Defrosting

Cleaning the Knife Holder

Specific instructions for cleaning the knife holder and removing wax build-up.

Defrosting the Cryotome

Procedure for defrosting the instrument to remove frost build-up.

Maintenance and Lamp Tube Removal

General Maintenance Procedures

General maintenance tasks, including switching off the instrument.

Removing the Lamp Tube

Steps to remove the fluorescent lamp tube from the instrument.

Lamp Tube Fitting and Electrical Protection

Fitting the Lamp Tube

Steps to install a replacement fluorescent lamp tube.

Electrical Protection System

Information on the instrument's electrical protection system and fault resets.

TROUBLE SHOOTING GUIDE

General Troubleshooting and Instrument Function

Overview of common issues, fault codes, and basic instrument function problems.

Sectioning Problems and Remedies

Sectioning Issues

Addresses common problems encountered during sectioning and their solutions.

Error Messages and Their Solutions

Instrument Error Messages

Lists various error messages, their possible causes, and recommended remedies.

SPECIFICATION AND ACCESSORIES

Instrument Specifications

Details physical dimensions, weight, and electrical specifications.

Power, Environment, and Part Numbers

Details power characteristics, environmental limits, and Thermo part numbers.

Instrument Accessories and Spares

Lists available instrument accessories and spare parts with part numbers.

Warranty Statement

Product Warranty Information

Information regarding the product warranty terms and conditions.

Declaration of Conformity

Compliance Directives and Standards

States the instrument's compliance with relevant CE directives and international standards.

Appendix A: Transportation Instructions

Introduction and Repacking Cryotome E

Guidance for transporting the instrument and step-by-step repacking for the E model.

Repacking the Standard Cryotome

Step-by-step guide for safely repacking the standard Cryotome model.

Product Return Safety Declaration

Decontamination Certificate and Return Guidelines

Form for certifying decontamination before return and checklist for returning instruments.

Appendix B: Approved Reagent List

Reagent and Cleaning Agent Lists

Lists approved decontaminants and cleaning agents for use with the Cryotome.

Appendix C: Examples

Suggested Cutting Temperatures for Frozen Tissues

Provides guideline cutting temperatures for various types of frozen tissue samples.