Time during which the reaction
is
followed.
Minimum total time is
5
s,
maximum
19
h
59
min
59
s.
TOTAL
TIME
parameter can be selected in mode 4.
Enter the total time as
I;DI.MM.SS,
OIfI
=
hours,
=seconds).
Any of the calculation modes can be applied to results from any of the measurement modes. Calculation
mcBe/mode-s
can
be
selected before or aRcr the measurement
is
done.
keys allow the results to
be
obtained later from earlier measured values.
The latest selected calculation mode is used until changed.
e is selected with the CALC MODE key. Tke list of calculation modes can
he.
scrolled
forwar
th
the
arrow
keys.
Calculation parameters ape selected with the CAeC
P
or ENTER key. The arrow or number keys are
used for entering new
parameters. Berault value or earlier determined value is selected by pressin
fie
ENTER
or CALC
The following calculation modes and their parameters can be selected in module
1:
I
NO CALCCR,ATION:
Results are sent
as
absorbance$ to built-in printer or via the interface.
2
FACTOR CKCULATION: Measured absorbances are multiplied with a factor given
by
the user
to get concentrations. The concentration unit is defined by the unit of
the factor. Concentration
is
calsulated according to the following
equation:
Concentration -FACTOR
x
absorbance.
The measurement parameters, calculation parameters and calculated
concentrations are sent to the built-in printer or via the interface.
Parameter: FACTOR
The user enters the concentrations of the standards and Multiskan
EX
calculates a standard line with the
help
of these standards using the
method of least squares. Measured absorbances are converted to
concentration units by the use of this line. The measurement
parameters, calculation parameters and concentrations obtained
are
sent to the built-in printer or via the interface.
Parameters:
Number of standards
O\iR
OF STDS)
Number of replicates
(NR
OF
REPE)
Standard concentrations
(STANDARD
1)
etc.
Standard locations
(STANDARD
L
REPL
1
Al)
els.
Up
to
8
nandard solution can incorporated on the plat% in up lo
3
replicates.
1
STB
ON
EVERY
PLATE
/
Standard on every plate
(1
STB
ON
EVERY
PLATE) or
2
RETAINEBSTD retained standard
(2
standard curve of the former plate
is
used) can be selected.
If
re
is selected
1
USE
OLD
1..
LIE
NOW will be asked before reading
the next plate.
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