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Amsco 250LS User Manual

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OPERATOR MANUAL
AMSCO
®
110LS and AMSCO
®
250LS
Life Sciences Small Sterilizers
(Rev A) P387366-389

Table of Contents

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Amsco 250LS Specifications

General IconGeneral
BrandAmsco
Model250LS
CategoryLaboratory Equipment
LanguageEnglish

Summary

Safety Precautions

Electric Shock and Burn Hazards

Warns about electrical hazards and proper lockout procedures for safety.

Personal Injury Hazards

Alerts to risks of injury from door operation and steam exposure.

Burn Hazards

Details risks associated with hot surfaces, steam, and hot fluids.

Explosion Hazards

Specifies that the sterilizer is not for flammable compounds.

Personal Injury and Equipment Damage Hazards

Emphasizes professional maintenance and authorized parts for safety.

Sterility Assurance Hazards

Highlights potential compromise of sterility if indicators show issues.

Installation Verification

2.1 Installation Checklist

A checklist to ensure correct and complete sterilizer installation.

Technical Specifications

Techniques of Sterilization

3.1 Recommended Sterilization Variables

Lists recommended variables for prevacuum, gravity, and liquid cycles.

3.1.1 Prevacuum Cycle Variables

Provides parameters for the prevacuum sterilization cycle.

3.1.2 Gravity Cycle Variables

Details parameters for gravity cycle sterilization of various items.

3.1.3 Liquid Cycle Variables

Specifies sterilization parameters for liquid volumes in containers.

3.2 Control Measures for Verifying Sterilization Process

Explains biological, chemical, and vacuum leak tests for validation.

3.2.1 Biological Monitors

Discusses the use of live spore tests for biological monitoring.

3.2.2 Chemical Measure (Bowie-Dick Tests)

Describes Bowie-Dick tests for verifying air removal in prevacuum cycles.

3.2.3 Vacuum Leak Test

Explains how to perform and interpret leak tests for system integrity.

3.4 Sterilization Techniques and Recommendations for Liquids

Details safe practices for sterilizing liquids, including container types.

Operator Instructions

4.1 Component Identification

Identifies major components and controls of the sterilizer.

4.2 Control Panel Overview

Explains the function of the control panel and its main interface.

4.2.1 Touch Screen Operation

Details how to use the touch screen for operating the sterilizer.

4.2.3 Operating Mode Selection

Explains how to select and start different operating modes.

Emergency Door Operation

Generator Flush Operation

Generator Flush Alarms

Control Interface

5.1 General Description

Introduces the touch screen interface for operating the sterilizer.

5.1.1 Log In Procedure

Details the steps for logging into the sterilizer system.

5.1.2 Password Entry

Explains how to enter the username and password for access.

5.2 Operating Modes

Explains how to select and start different sterilizer operating modes.

5.2.1 Prevac Sterilizer Only Mode

Details how to select and run the Prevac sterilization cycle.

5.2.2 Isothermal Sterilizer Only Mode (Optional Cycle)

Describes how to select and run the optional Isothermal cycle.

Sterilizer Operation

6.1 Before Operating Sterilizer

Lists essential steps and safety checks before operating the unit.

6.1.1 Load Sterilizer Procedure

Details the process of loading items into the sterilizer chamber.

6.2 Gravity Cycle Operation

Provides instructions for running the Gravity cycle.

6.3 Prevac Cycle Operation

Details the procedure for running the Prevac cycle.

6.4 Liquid Cycle Operation

Guides on running the Liquid cycle for sterilizing liquids.

6.20 Supervisor Mode Functions

Covers various functions accessible within Supervisor Mode.

6.20.1 Cycle Values Configuration

Guides on viewing and changing cycle parameters.

6.21 21 CFR Part 11 Compliance

Explains features for record retention, traceability, and electronic signatures.

Alarms

IN CYCLE ALARMS – Non-Aborting

Lists alarms that occur during a cycle but do not stop it.

IN CYCLE ALARMS – Aborting

Lists alarms that occur during a cycle and cause it to abort.

Routine Maintenance

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