EF600 Instruction Manual
Asymptote Ltd.
St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK
Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk
same time as the ‘Cycle On’ light. It will switch off automatically when the
temperature starts to decreases.
5.7 Sample nucleation
When the samples reach a programmed hold at -7 ºC (the nucleation plateau) a
buzzer sounds and the ‘Nucleate Now’ yellow light illuminates. Nucleation
must be done at this time. The program allows a 10 minute period for the
samples to be nucleated, unless otherwise specified.
Remove the lid of the freezer completely. Touch the nucleating tool (if using
the cryopen please refer to Appendix 4 for operating instructions) to the first
straw for approximately 1 second (figure 5.7). Repeat with the remaining
straws and close the lid of the freezer. Turn off the buzzer by pressing the
Inspect the straws approximately one minute after nucleation to verify that
there is some ice-growth in the straws. If ice has not formed in any of the
straws repeat nucleation.
Figure 5.7 – Nucleation of straws
When the cycle is complete the yellow ‘Cycle Complete’ light will illuminate.
The straws can now be removed from the freezer and placed in storage. The
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General