EF600 Instruction Manual
Asymptote Ltd.
St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK
Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk
1. INTRODUCTION ................................................................................................ 5
1.1 WARNING .............................................................................................................. 6
1.2 SAFETY PRECAUTIONS ........................................................................................... 6
1.3 MAINTENANCE ....................................................................................................... 6
2. INSTALLING THE FREEZER UNIT ............................................................... 7
2.1 UNPACKING ........................................................................................................... 7
2.2 POSITIONING THE FREEZER .................................................................................... 7
2.3 ELECTRICAL CONNECTION .................................................................................... 7
2.4 SWITCHING THE FREEZER ON ................................................................................. 7
4. USER INTERFACE (EF600-01, EF600-10) ..................................................... 10
4.1 SELECTING THE LOGGING INTERVAL (EF600-01, EF600-10) AND FREEZING
PROFILE (EF600-10) ............................................................................................ 10
4.2 LOG FILE NAMES (EF600-01, EF600-10) ............................................................. 11
5. OPERATING PROCEDURE FOR THE EF600 FREEZER ......................... 13
5.1 PRE-OPERATION CHECKS ...................................................................................... 13
5.2 PREPARING THE NUCLEATING TOOL ..................................................................... 13
5.3 PREPARING THE STRAWS ...................................................................................... 13
5.4 LOADING THE STRAWS ........................................................................................ 13
5.5 STARTING THE DATA LOGGING (EF600-01, EF600-10) ....................................... 14
5.6 STARTING THE FREEZING CYCLE .......................................................................... 14
5.7 SAMPLE NUCLEATION .......................................................................................... 15
5.8 END OF CYCLE ..................................................................................................... 15
5.9 STOPPING THE DATA LOGGING ............................................................................. 16
5.10 RESTARTING THE CYCLE ...................................................................................... 16
5.11 CLEANING THE UNIT ............................................................................................ 16
6. FREEZING CYCLES ........................................................................................ 17
6.1 HUMAN EMBRYO CRYOPRESERVATION ................................................................ 17
6.2 HUMAN OOCYTE CRYOPRESERVATION ................................................................. 17
6.3 HUMAN BLASTOCYST CRYOPRESERVATION ......................................................... 18
6.4 HUMAN EMBRYONIC STEM CELL CRYOPRESERVATION ......................................... 18
6.5 HUMAN SPERMATOZOA CRYOPRESERVATION – LINEAR COOLING ........................ 19
6.6 HUMAN SPERMATOZOA CRYOPRESERVATION – NON-LINEAR COOLING ................ 20
7. REFERENCES .................................................................................................... 21
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