Asymptote EF600 Series User Manual

EF600 Instruction Manual
Asymptote Ltd.
St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK
Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk
20
6.6 Human spermatozoa cryopreservation – non-linear cooling
• Start temperature 20°C
• Cool from 20°C to -7°C at 2°C min
-1
• Hold for nucleation at -7°C for 10 minutes
• Cool non-linear from -7°C to -100°C at an
approximate rate of 10°C min
-1
• Hold at -100°C
-100
-80
-60
-40
-20
0
20
00:00 00:10 00:20 00:30 00:40
Time (hh:mm:ss)
Temperature (°C)
Figure 6.6 – Pre-programmed human spermatozoa non-linear freezing cycle

Asymptote EF600 Series Specifications

General

Brand	Asymptote
Model	EF600 Series
Category	Freezer
Language	English