EF600 Instruction Manual
Asymptote Ltd.
St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK
Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk
• Start temperature 20°C
• Cool from 20°C to -7°C at 2°C min
-1
• Hold for nucleation at -7°C for 10 minutes
• Cool from -7°C to -80°C at 1°C min
-1
• Hold at -80°C
-80
-60
-40
-20
0
20
00:00 00:20 00:40 01:00 01:20 01:40 02:00
Figure 6.4 – Pre-programmed human embryonic stem cell freezing cycle
6.5 Human spermatozoa cryopreservation – linear cooling
• Cool from 20°C to -7°C at 2°C min
-1
• Hold for nucleation at -7°C for 10 minutes
• Cool from -7°C to -100°C at 10°C min
-1
-100
-80
-60
-40
-20
0
20
00:00 00:10 00:20 00:30 00:40 00:50
Figure 6.5 – Pre-programmed human sperm freezing cycle