Asymptote EF600 Series User Manual

EF600 Instruction Manual
Asymptote Ltd.
St John’s Innovation Centre, Cowley Road, Cambridge, CB4 0WS, UK
Tel: +44 1223 421161, Fax: +44 1223 421166, E-mail: info@asymptote.co.uk
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6. Freezing cycles
The freezing cycles correspond to the standard set of freezing programs pre-
loaded in the EF600 freezer. Unless otherwise requested, equipment pre-
loaded with a single program (EF600-00 and EF600-01) will run the standard
profile for human embryo cryopreservation (section 6.1). The EF600-10
freezer is loaded with all six profiles below (sections 6.1 to 6.6).
6.1 Human embryo cryopreservation
• Start temperature 20°C
• Cool from 20°C to -7°C at 2°C min
-1
• Hold for nucleation at -7°C for 10 minutes
• Cool from -7°C to -30°C at 0.3°C min
-1
• Cool from -30°C to -100°C at 10°C min
-1
• Hold at -100°C
-100
-80
-60
-40
-20
0
20
00:00 00:20 00:40 01:00 01:20 01:40 02:00
Time (hh:mm)
Temperature (°C)
Figure 6.1 – Pre-programmed human embryo freezing cycle
6.2 Human oocyte cryopreservation

Asymptote EF600 Series Specifications

General

Brand	Asymptote
Model	EF600 Series
Category	Freezer
Language	English