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BD FACSFlow Supply System - Page 41

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Chapter 4: Maintenance 41
Attaching the new
filter
To attach the new filter:
1. Connect the vent line to the new filter assembly.
Twist to attach.
2. Wrap Teflon® tape around the filter threads, then connect the
filter to the filter base.
3. Connect the sheath line to the filter assembly by squeezing the
quick-disconnect.
4. Attach the cytometer fluid line to the filter assembly via the
quick-disconnect.
5. Install the filter assembly into the holder on the sheath plenum
enclosure.
6. Direct the vent line into a beaker and loosen the vent cap until
a steady stream of fluid empties from the filter.
7. Verify that no air is trapped in the filter.
8. Repeat steps 6 and 7 as necessary to remove all trapped air.