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Optima XPN
Beckman Coulter Optima XPN User Manual
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PN B08115AF
February 2018
User Guide
Optima XPN Series
Preparative Ultracentrifuges
Beckman Coulter, Inc.
250 S. Kraemer Bl
vd.
Brea, CA 92821 U.S.A.
2
Table of Contents
Default Chapter
3
Revision History
3
Alerts for Danger, Warning, Caution, Important, and Note
5
Safety and Notices
5
Safety Notices
6
Information
6
Installation
6
Replacement Parts
7
Service
7
Power Supply
7
Fuse Replacement
7
Mechanical Safety
8
Precautions with Liquids
9
Volatile Liquids
9
Certification
9
Scope of Manual
9
CFC-Free Centrifugation
10
Software Copyright
10
Rohs Notice
11
China Rohs Caution Label
11
China Rohs Environmental Label
11
Summary of Instrument Labels
12
Caution Symbol
12
Universal Serial Bus (USB)
12
Ethernet
12
Recycling Label
13
Multiple Compliance
13
CAUTION Static Sensitive Area
14
DANGER High Voltage
14
Protective Ground
14
Output Plate Label
15
Rotor Rotation
15
Symbols
16
Table of Contents
17
Tab
29
CHAPTER 1 Description
31
Name Rating Plate
31
Rotor Chamber
31
Touch Screen
31
Vacuum System
31
Drive
32
Safety Features
32
Door
32
Barrier Ring
33
Imbalance Detector
33
Overspeed System
33
Dynamic Rotor Inertia Check (DRIC)
33
Temperature Sensing and Control
32
Specifications
33
Control Features
34
Physical Data
36
Audible Sounds
37
Available Rotors
38
CHAPTER 2: The Touch Screen Interface
39
Areas on the Screen
39
Header Bar
39
Button
39
Menu Button
40
Status Display
40
Ready Status
40
Help Button
41
Footer Bar
41
Header Bar - Ready Status
41
Header Bar - Running Status
41
Header Bar - Stopping Status
41
Header Bar - Warning Message
41
Header Bar - Error Message
41
Start Button
42
Stop Button
42
Footer Bar on the Home Page
43
Footer Bar on Other Pages
43
Vacuum Display/Button
43
System Name
43
Footer Bar - Home Page
43
Accel and Decel Display/Button
44
Page Display Area
44
Help Messages
44
Item Help
44
Item Help Button
44
Global Help
45
Global Help Button Attached to Help Button
45
Home Page Help with Ghost Image
45
Transparency Button
45
System References
46
Home Page Help with Opaque Screen
46
Help Navigation Buttons
46
About Your Instrument
47
References Page
47
About Page
48
CHAPTER 3: Operations
49
Manual Operation
49
Step 1: Start on the Home Page
50
Step 2: Set the Speed and Rotor
51
Step 3: Set the Acceleration and Deceleration Profiles
53
Step 4: Set the Time
54
Set Time Page
54
Step 5: Set the Temperature
55
Set Time Page
55
Delay Start Page
55
Set Temperature Page
56
Step 6: Start the Run
57
Start Button
57
Zonal and Continuous Flow Operation
58
Zonal Page
58
Preparing for the Run
59
Starting the Run
59
Loading the Sample
60
Running the Sample
60
Unloading the Sample
60
Continuous Flow Page
60
Stopping the Run
61
Finishing the Run
61
CHAPTER 4 Configuration
63
Managing Your Network
63
Setting up the Network
63
Selecting a Printer
64
Setting up Email
65
Setting up VNC
66
Enabling API
66
Managing Users
67
Adding Users
67
Requiring Login
68
PIN Expiration and Logout Timer
69
User Options
70
Managing Rotors
70
Adding Rotors
71
Requiring Rotor Selection
71
Managing Reports
72
User Access
72
Filter Data
72
Run History Filter
73
Set from Date and Time
73
Graph Data
74
Run Graph Button
74
Print Data
75
Run History Button
75
Run Graph Options
75
Export Data
76
Run Graph Button
76
Run History Button
76
Run History Button
77
Auto Print and Auto Export Run History Data
78
To Enable Auto Print
78
To Enable Auto Export
78
Run Comments
79
E-Signature
80
Before Run Comment
80
Signature
81
CHAPTER 5 Programs
83
Creating Programs
83
Program Button
83
Select Program
83
Unnamed Program
84
New Program Name
85
Set Acceleration/Deceleration Profiles
85
New Step
86
Running Programs
87
New Step in a Numbered Sequence
87
Program Button
87
Editing Programs
88
Deleting Programs
88
Program Button
88
CHAPTER 6: Calculations
91
Using Calculations
91
Calculations Page
91
Reduce Rotor Speed for Dense Solutions
93
Reduce Rotor Speed for Precipitating Solutions
94
Sedimentation Coefficient from Run Data
95
Sedimentation Coefficient from Molecular Mass
97
Pelleting Time
98
Pelleting Time Page
98
Refractive Index
99
Concentration Measures
100
Calculate Refractive Index Page
100
Calculate Concentration Measures Page
101
CHAPTER 7: Simulations
103
Using Simulations
103
Simulations Page
103
ESP RNA Pelleting Best Run
105
Simulations Page
105
ESP RNA Pelleting in Csci with Guscn, Optimized for Purity
106
ESP RNA Pelleting Fast Run
107
ESP RNA Pelleting in Csci with Guscn, Optimized for Speed
107
ESP Pelleting Run
108
ESP Pelleting Separation Page
109
ESP Rate Zonal Run
110
ESP Plasmid Run
111
ESP Optimized Plasmid DNA Separation Page
112
Substitute Rotor Run
113
Substitute Rotor Run Page
113
CHAPTER 8 Pages
115
Reduce Rotor Speed for Dense Solutions Page
116
Reduce Rotor Speed for Precipitating Solutions Page
116
Determine Sedimentation Coefficient from Run Data Page
116
Determine Sedimentation Coefficient from Molecular Mass Page
116
Home Page
117
Home Page (Run in Progress)
119
Set Speed Page
120
Home Page
120
Select Rotor and Labware Page
121
Set Time Page
122
Delay Start Page
123
Set Speed Ω²T Time Page
124
Select Rotor and Labware Page
124
Set Temperature Page
125
Set Acceleration/Deceleration Profiles Page
126
Home Page
126
Example Acceleration and Deceleration Profiles
127
Login Page
128
Select Program Page
129
New/Edit/View Program Page
130
New Program Page
130
Edit Program Page
130
View Program Page
131
Select Rotor and Labware Page
132
Select Rotor and Labware Page (Catalog)
132
New/Edit Step Page
133
New Step Page
133
Edit Step Page
133
Program Log Page
134
Export Page
135
Authorize Users Page
136
Import Page
137
Real-Time Run Data/Historical Run Data Page
138
Button
138
Real-Time Run Data Page
138
Historical Run Data Page
138
Run Graph Options Page
139
Run Graph Button
139
Run History Page
140
Run History
141
Run History Button
141
User Levels
141
Run History Filter Page
142
Before Run/After Run Comment Page
143
Menu Page
144
System Options Page
145
The Basic Tab
145
System Options Page, Basic
145
The System Tab
146
System Options Page, System
146
The Network Tab
147
System Options Page, Network
147
The Users Tab
148
System Options Page, Users
148
The Reports Tab
149
System Options Page, Reports
149
Select Language Page
150
User Options Page
151
Reset User PIN Page
152
Select Image Page
153
Pn B08115Af
153
Set Date and Time Page
154
System Log Page
155
Manage Rotors Page
156
Add to Rotor Library Page
157
Add to Rotor Library
157
Diagnostic History Page
158
Set Sound Page
159
Custom Sounds Page
160
Archive Data Page
161
Setup Network Page
162
The Network Tab
162
Select Printer Page
163
Setup Email Page
164
Setup VNC Page
165
Manage Users Page
166
Add/Edit User Page
167
Add User Page
167
Authorize Programs Page
168
Authorize Programs
168
References Page
169
Rotor Catalog Page
170
Compatible Tubes for Rotor Page
171
Compatible Tubes for Rotor
171
Labware Catalog Page
172
Chemical Resistances Page
173
References Page
173
Chemical Resistance
173
Reduce Rotor Speed for Dense Solutions Page
175
Reduce Rotor Speed for Precipitating Solutions Page
176
Determine Sedimentation Coefficient from Run Data Page
177
Determine Sedimentation Coefficient from Molecular Mass Page
178
Calculate Pelleting Time Page
179
Calculate Concentration Measures Page
180
Calculate Refractive Index Page
181
About Page
182
Zonal/Continuous Flow Authorization Page
183
Menu Page
183
Zonal Authorization Page
183
Zonal Mode Button
183
Zonal/Continuous Flow Operation Page
184
Zonal Operation Page
184
Continuous Flow Operation Page
184
Simulations Page
186
ESP Rate Zonal Separation Page
186
Run
186
ESP RNA Pelleting in Cscl with Guscn, Optimized for Purity Page
187
ESP RNA Pelleting in Cscl with Guscn, Optimized for Purity
187
Select Rotor and Labware Page
188
Select Rotor and Labware Page (Catalog/Library)
188
(Catalog/Library)
189
ESP RNA Pelleting in Cscl with Guscn, Optimized for Speed Page
189
Select Rotor and Labware Page
189
ESP RNA Pelleting in Cscl with Guscn, Optimized for Speed
189
ESP Pelleting Separation Page
190
Select Rotor and Labware
191
ESP Optimized Plasmid DNA Separation Page
192
Select Rotor and Labware Page
192
ESP Rate Zonal Separation Page
193
Substitute Rotor Run Page
194
Menu Page
115
Export Page
115
Select Image Page
115
CHAPTER 9: Maintenance and Troubleshooting
197
Field Service
197
Rotors and Labware
197
Cleaning
197
Instrument Surfaces
197
Rotor Chamber
198
Chamber Door O-Ring
198
Decontamination
198
Sterilization and Disinfection
198
Diagnostics/User Messages
199
Retrieving Your Sample in Case of Power Failure
199
During a Run
199
Rotor Spinning at Restoration
199
Rotor Stopped at Restoration
199
Getting Access to the Sample
200
Panel Screws and Tabs
201
Internal Parts - Configuration "A" Vents through the Port Cap
202
Internal Parts - Configuration "B" Vents through the Vacuum Solenoid Release Screw
203
Restoring the Instrument to Operating Condition
205
Storage and Transportation
206
Supply List
207
Replacement Parts
207
Supplies
207
APPENDIX A: Preinstallation Requirements
209
Overview
209
Space Requirements
209
Safety
209
Ventilation
210
Temperature
210
Electrical Requirements
210
Safety and Ventilation Space
210
Electrical Connection
211
A.2 Electrical Connection
211
APPENDIX B: Special Warranty for the Optima XPN
213
Special Warranty
213
APPENDIX C: Diagnostics
215
Overview
215
Diagnostics/User Messages Chart
215
APPENDIX D: Third Party Contributions and Legal Notices
223
4
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Beckman Coulter Optima XPN Specifications
General
Brand
Beckman Coulter
Model
Optima XPN
Category
Laboratory Equipment
Language
English
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