Beckman Coulter Optima XPN User Manual

PN B08115AF
February 2018
User Guide
Optima XPN Series
Preparative Ultracentrifuges
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

Beckman Coulter Optima XPN Specifications

General

Type	Ultracentrifuge
Max Speed	150, 000 rpm
Control Interface	Touchscreen
Temperature Control	Yes
Vacuum System	Yes
Power Requirements	220-240 V, 50/60 Hz

Summary

Safety and Notices

Alerts for Danger, Warning, Caution, Important, and Note

Defines standard safety alert symbols and their meanings.

Safety Notices

General safety precautions before installing, using, or maintaining the instrument.

Summary of Instrument Labels

Provides information on labels and symbols appearing on the instrument housing.

CHAPTER 1: Description

Touch Screen

Describes the touch screen interface for instrument control and information display.

Specifications

Lists guaranteed and informative data with tolerances or limits for instrument performance.

Safety Features

Notes the instrument's safety design for indoor operation at specified altitudes.

Imbalance Detector

Details the system that monitors rotor balance and initiates automatic shutdown.

Available Rotors

Information on compatible Beckman Coulter floor-model ultracentrifuge rotors.

CHAPTER 2: The Touch Screen Interface

Areas on the Screen

Identifies the three main areas on the touch screen: Header Bar, Footer Bar, and Page Display Area.

Footer Bar

The bottom area of the touch screen containing Start and Stop buttons, and other context-specific controls.

Help Button

Provides access to built-in online help messages for instrument operation.

System References

Provides access to reference materials like Rotor Catalog, Labware Catalog, and Chemical Resistances.

CHAPTER 3: Operations

Manual Operation

Guides through basic run procedures using the Home Page interface.

Zonal and Continuous Flow Operation

Details procedures for specialized runs involving sample loading/unloading while the rotor spins.

CHAPTER 4: Configuration

Managing Your Network

Options for connecting the instrument to a network, enabling email, file transfer, and VNC.

Managing Users

Setting up user accounts, levels of access, and login requirements for system control.

Managing Reports

Options for saving, filtering, graphing, printing, and exporting run history data.

Auto Print and Auto Export Run History Data

Configuration for automatic printing or network export of run history data.

CHAPTER 5: Programs

Creating Programs

Steps to create, name, and define parameters for new run programs.

Running Programs

How to select and start a previously created or saved program.

CHAPTER 6: Calculations

Reduce Rotor Speed For Dense Solutions

Calculates reduced run speed for solutions denser than rotor rating to prevent stress.

Sedimentation Coefficient From Run Data

Calculates sedimentation coefficient using data from a previous run.

Pelleting Time

Calculates the minimum time required to pellet a particle.

CHAPTER 7: Simulations

Using Simulations

Explains how to create and use simulations to optimize run conditions and predict separations.

ESP RNA Pelleting Best Run

Simulates RNA separation optimized for purity, considering CsCl and GuSCN gradients.

ESP Pelleting Run

Simulates particle separation and predicts the shortest possible pelleting time.

CHAPTER 8: Functional Pages

Home Page

The primary interface page for instrument startup and initiating most operations.

Set Speed Page

Allows setting the rotor speed in RPM or RCF units for the next run.

Set Time Page

Allows setting the run duration in hours and minutes, including hold modes.

Set Acceleration/Deceleration Profiles Page

Enables selection of programmed acceleration and deceleration profiles.

System Options Page

Main page for configuring system settings, organized into five tabs.

Calculations Page

Access point for various calculations useful in ultracentrifugation.

Simulations Page

Access point for running computer simulations of centrifugation runs.

CHAPTER 9: Maintenance and Troubleshooting

Field Service

Contact information and procedures for obtaining maintenance assistance from Beckman Coulter.

Cleaning

Recommended methods and materials for cleaning instrument surfaces and components.

Diagnostics/User Messages Chart

A comprehensive chart listing error codes, their meanings, and recommended actions.

Retrieving Your Sample in Case of Power Failure

Procedures for accessing the sample if power is lost during operation.

APPENDIX A: Preinstallation Requirements

Safety

Crucial safety information, including electrical connection and placement guidelines.

Electrical Requirements

Details power requirements, voltage, frequency, and current ratings.

APPENDIX B: Special Warranty for the Optima XPN

Special Drive Warranty

Details warranty coverage and replacement pricing for the instrument's drive unit.

APPENDIX C: Diagnostics

Diagnostics/User Messages Chart

A comprehensive chart listing error codes, their meanings, and recommended actions.