Beckman Coulter CytoFLEX User Manual (English)

Questions and Answers:

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Brett AtkinsAug 16, 2025
How to fix a Beckman Coulter CytoFLEX Cytometer that will not turn on?
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  Adam BarkerAug 16, 2025
  If the Cytometer cannot be turned on, it might be due to the instrument being turned off in the Cytometer menu, the power switch being in the off position, the power cable not being securely connected, or a blown fuse. Here’s how to address it: 1. Ensure the power switch is in the on position on the back of the Cytometer. 2. Select Turn On in the Cytometer menu. 3. Ensure that the power cable is securely connected to the back of the Cytometer. 4. Replace the fuse.
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deborahtuckerAug 19, 2025
What to do if my Beckman Coulter Laboratory Equipment Workstation won't turn on?
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  Tiffany FoxAug 19, 2025
  If the Workstation cannot be turned on, it may be because the power cable is not securely connected or the Workstation was restarted too fast. To solve this: 1. Ensure that the power cable is securely connected to the back of the Cytometer. 2. Unplug the power cable, wait 10 seconds, then plug it back in. Then, restart the computer.
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rlamAug 22, 2025
How to fix Beckman Coulter Laboratory Equipment sample tube holder that won't move automatically?
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  Donna CraigAug 22, 2025
  If the sample tube holder on your Beckman Coulter Laboratory Equipment cannot move up and down automatically, the setting might be incorrect. Ensure that the sample injection mode in the software is in Semi-Automatic Injection mode.
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Anita TaylorAug 25, 2025
How to resolve low laser power on Beckman Coulter Laboratory Equipment?
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  Keith PotterAug 26, 2025
  If the laser power is low on your Beckman Coulter Laboratory Equipment, it may be due to a communication error. To resolve this: 1. Reinitialize. 2. Restart the Cytometer.
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Lisa MatthewsAug 28, 2025
What to do if the Beckman Coulter CytoFLEX shows a Disconnected and Error message?
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  Vanessa MartinezAug 28, 2025
  If the connection indicator light in the lower left corner of the software screen is red and displays Disconnected and Error, it could be due to a data connection error, the Cytometer not being turned on, or the Cytometer’s power cable being disconnected. To resolve this: * Ensure the USB data cable is securely connected to the back of the Cytometer and the Workstation. * Restart the software and the Workstation. * Turn on the Cytometer using the power switch on the back of the instrument. * Verify that the power cable is securely connected to the back of the Cytometer.
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yhughesAug 31, 2025
Why is the alarm not working on my Beckman Coulter CytoFLEX Laboratory Equipment?
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  michaeljeffersonAug 31, 2025
  If the alarm does not sound when the waste container is full or the sheath fluid container is low and the software status display is red, it could be due to the alarm not working, an instrument data connection error, the sheath fluid/waste harness float being restricted, or the sheath fluid/waste harnesses being secured on the wrong container. To resolve this: 1. Ensure the sheath fluid/waste harnesses are secured to the correct container. 2. Ensure that the USB data cable is securely connected to the back of the Cytometer and the back of the Workstation. 3. Restart the Workstation. 4. Restart the software. 5. Verify that the float of the sensor in the sheath fluid/waste container moves freely. 6. Replace the sheath fluid/waste harness.
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Shannon MyersSep 6, 2025
Why is the sample flow rate unstable on my Beckman Coulter Laboratory Equipment?
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  Shannon MeyerSep 6, 2025
  If the sample flow rate is unstable on your Beckman Coulter Laboratory Equipment, it could be due to several reasons: * The sample probe is clogged. * The sample contains aggregates or clumps. * There are air bubbles in the flow cell. * The sample peristaltic pump tubing is aged or not properly connected. To address this: 1. Run Prime. 2. Run Daily Clean. 3. Clean the sample probe. 4. Filter the sample using an appropriately sized mesh aperture filter. 5. Ensure that the sample tubing is properly connected. 6. Replace the sample probe and sample peristaltic pump tubing.
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Taylor FritzSep 9, 2025
How do I stop populations from drifting on my Beckman Coulter Laboratory Equipment?
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  Sean RuizSep 9, 2025
  If populations are drifting on your Beckman Coulter Laboratory Equipment, it could be due to air bubbles in the flow cell or system, or the sheath fluid harness float being restricted. To resolve this, you can: 1. Run Prime. 2. Ensure that the sheath fluid harness and/or waste harness is not kinked and is securely connected. 3. Run Prime again. 4. Verify that the float of the sensor in the sheath fluid container moves freely. 5. Replace the sheath fluid harness.

Beckman Coulter CytoFLEX Specifications

General

Detection Channels	Up to 13 fluorescence channels
Sample Volume	12 µL to 1, 000 µL
Side Scatter (SSC)	Yes
Data Acquisition Rate	Up to 30, 000 events per second
Software	CytExpert
Sample Volume Options	12 µL to 1, 000 µL
Power Requirements	100-240 V, 50/60 Hz
Lasers	Up to 3 lasers (405 nm, 488 nm, 561 nm, 638 nm)