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Beckman Coulter CytoFLEX SRT User Manual

Beckman Coulter CytoFLEX SRT
393 pages
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C37808AC
March 2022
Instructions for Use
CytoFLEX SRT
Cell Sorter
For Research Use Only. Not for use in diagnostic procedures.
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821 U.S.A.

Table of Contents

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Beckman Coulter CytoFLEX SRT Specifications

General IconGeneral
TypeFlow Cytometer
Forward Scatter (FSC)Yes
Side Scatter (SSC)Yes
SoftwareCytExpert SRT Software
Operating Temperature15-30°C (59-86°F)
Laser ConfigurationUp to 4 lasers
Data Acquisition RateUp to 30, 000 events/second
Particle Size Detection0.2 µm to 40 µm
Power Requirements100-240 VAC, 50/60 Hz
Operating Humidity20% to 80% non-condensing
Weight45 kg (100 lbs)

Summary

Instructions for Use

Product Overview

Introduces the CytoFLEX SRT Cell Sorter, its purpose, and configurations.

Safety Information

Details critical safety notices, warnings, precautions, and symbol explanations.

Introduction

Manual Overview and Conventions

Explains manual organization, usage guidelines, conventions, and graphic representations.

Chapter 1: System Overview

System Overview and Principles

Describes the overall system, product details, and core operation principles.

Instrument Components

Details fluidics cart, sorter, workstation, fluidics system, sample station, and optical components.

Instrument Specifications and Performance

Covers dimensions, electrical ratings, altitude, noise, and performance characteristics.

Chapter 2: Using the CytExpert SRT Software

Software Interface and Navigation

Covers launching software, main screen, start page, acquisition, and analysis screens.

Software Menus and User Management

Explains software menus, user/role management, policies, and operation logs.

Software Settings and Libraries

Details sort mode library, collection device library, and software configuration settings.

Chapter 3: Daily Startup

Instrument Startup Procedures

Outlines pre-startup checks, powering on, logging in, and system startup program execution.

Chapter 4: Instrument Quality Control and Standardization

Quality Control Process

Explains QC workflow, sample preparation, data collection, and result confirmation.

Standardization Process

Details sample preparation, target value generation, performing standardization, and applying settings.

Chapter 5: Sorting

Experiment Setup and Configuration

Covers experiment creation, tube/plate setup, sort modes, and acquisition settings.

Sorting Execution and Monitoring

Details starting/monitoring sorts, side stream monitor, auto maintain, and statistics.

Data Export and Reporting

Covers exporting plots, statistics, FCS files, and generating sort reports.

Chapter 6: Compensation

Compensation Experiment Setup

Describes creating compensation experiments, preparing samples, and generating matrices.

Adjusting and Managing Compensation

Covers manual adjustment, import/export settings, and compensation library management.

Chapter 7: Data Review

Data Analysis Workflow and Plotting

Covers copying/importing data, analysis screen, plots, statistics, and overlays.

Sample Concentration and Adjustments

Details calculating sample concentration and adjusting compensation settings.

Chapter 8: Shutting Down the System

Shutdown Procedures

Covers daily clean, system shutdown, long-term shutdown, cleaning, and powering off.

Chapter 9: Troubleshooting

Laser and Hazard Safety

Details laser hazards, warning labels, biohazard, electrical, and pinch hazards.

Troubleshooting Guide

Provides a table of common problems, causes, and corrective actions for error codes.

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