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COUL
TER HmX Hematology Analyzer
COUL
TER HmX Hematology Analyzer with
Autoloader
Operator’
s
T
raining
Guide
PN
4237530BB (March
2011)
Beckman Coulter
, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821
AVOID EXPOSURE, LASER RADIATION
EMITTED FROM THIS APERTURE
B
ECKMAN
C
OULTER
HmX
COULTER
HmX
®
B
ECKMAN
C
OULTER
HmX
COULTER
HmX
®
2
Table of Contents
COULTER HmX Hematology Analyzer
1
COULTER HmX Hematology Analyzer with Autoloader
1
REVISION STATUS
3
CONTENTS
5
INTRODUCTION
7
PURPOSE OF THIS DOCUMENT
7
DOCUMENTATION
7
HmX Hematology Analyzer with Autoloader
7
HmX Hematology Analyzer
7
ABOUT THIS TRAINING GUIDE
8
Scope
8
Topics
8
Organization
8
Summary Pages
9
Training Checklist
9
RESPONSIBILITIES
9
Beckman Coulter’s Responsibility
9
Key Operator’s Responsibility
9
CONVENTIONS
10
GRAPHICS
10
GETTING TO KNOW YOUR INSTRUMENT
11
OBJECTIVES
11
When the subject is complete, you will be able to . . .
11
Data Management System (DMS)
11
Main Unit
11
Modes of Operation
11
Reagents
11
ACCESS SCREEN
12
SOFTWARE MENU TREE - HmX Hematology Analyzer with Autoloader
13
SOFTWARE MENU TREE - HmX Hematology Analyzer
14
STATUS LINE TABLE
15
DATA MANAGEMENT SYSTEM (DMS)
16
Functions of the Data Management System (DMS)
16
Major Components
16
Computer
16
Monitor
16
Printer
16
Keyboard
16
Layout
17
Hot Keys
18
Screen Conventions
19
Menu Item Conventions
19
Display Screen Conventions
19
Instructional and Informational Message Convention
19
MAIN UNIT - HmX Hematology Analyzer
20
Front View of the Main Unit
20
Controls and Indicators
20
POWER On/Off Rocker Switch
20
Standby/Reset Rocker Switch
20
Ready Indicator Light
21
Standby Indicator Light
21
Entry Port
21
Exit Tray
21
Aspirator Probe
21
Sample Bar
21
Bar-code Reader
22
Green LED
22
Red LED
22
HmX Hematology Analyzer . . . a Single-Specimen, Rotary Cap-Pierce System
22
Primary Mode
22
Secondary Mode
23
Predilute Mode
23
Retic Mode
23
MAIN UNIT - HmX Hematology Analyzer
24
Modules on the Front of the Main Unit
24
Modules on the Front of the Main Unit
25
Analyzer Module
25
Electronic Power Supply
25
Rotary Cap-Pierce Module
25
CBC Module
25
BSV (Blood Sampling Valve) Module
25
Pump Module
25
Right Side of the Main Unit
26
Modules on the Right Side of the Main Unit
26
Main Diluter Module
26
Flow Cell Module
26
Mixing Module
26
Back View of the Main Unit
27
Module on the Back of the Main Unit
27
Pneumatic Power Supply
27
MAIN UNIT - HmX Hematology Analyzer with Autoloader
28
Front View of the Main Unit
28
Controls and Indicators
28
Power On/Off Rocker Switch
28
Standby / Reset Rocker Switch
29
Ready Indicator Light
29
Standby Indicator Light
29
Loading Bay
29
Aspirator Probe
29
Sample Bar
29
Emergency Stop Button
29
HmX Hematology Analyzer with Autoloader
30
Primary Mode
30
Secondary Mode
30
Predilute Mode
31
Retic Mode
31
MAIN UNIT - HmX Hematology Analyzer with Autoloader
32
Modules on the Front of the Main Unit
32
Modules on the Front of the Main Unit
33
Analyzer Module
33
Electronic Power Supply
33
Autoloader Module
33
CBC Module
33
BSV (Blood Sampling Valve) Module
33
Pump Module
33
Right Side of the Main Unit
34
Modules on the Right Side of the Main Unit
34
Main Diluter Module
34
Flow Cell Module
34
Mixing Module
34
Back View of the Main Unit
35
Module on the Back of the Main Unit
35
Pneumatic Power Supply
35
REAGENTS
36
Reagents Connected to the Main Unit
36
Special Reagents Used in Sample Preparation for Retic Analysis
37
Storage and Handling
37
1 Record the New Reagent Information
37
2 Replace the Reagent Container
38
3 Prime the Lines
38
1 Put the Instrument into Standby
38
2 Turn OFF the Main Power
38
3 Replace the Waste Container
38
4 Turn ON the Main Power
38
5 Put the Instrument into Ready
38
STARTUP / SHUTDOWN
39
OBJECTIVES
39
When the subject is complete, you will be able to . . .
39
Startup
39
Clean Cycle
39
Shutdown
39
STARTUP
41
Summary Screen
41
Reagent Summary
41
System Check Summary
41
System Status Values Screen
42
Background Values
42
System Values
42
1 Check Current Instrument Status
43
2 Perform Start Up
43
3 Review Results
43
4 Perform Quality Control Checks
43
SHUTDOWN
45
Clean Cycle
45
Shut Down
45
1 Perform the Clean Cycle
45
2 Let the Instrument Stand
45
3 Review Results
45
4 Perform Quality Control Checks
45
1 Perform Shut Down
46
2 Let the Instrument Stand
46
SET UP OPTIONS
47
OBJECTIVES
47
When the subject is complete, you will be able to . . .
47
SET UP MENU OPTIONS
49
CONTROL SET UP
49
Setting Up Your Laboratory’s Control Files
49
Setting Up a LATRON Control File
50
1 Select the File
50
2 Type Manual Entries
50
3 Verify Host Setting
50
4 Verify All Information
50
Setting Up a 5C Cell Control File
50
1 Select the File
50
2 Upload the Assay Values
50
3 Type Manual Entries
50
4 Verify Host Setting
50
5 Verify All Information
50
6 Set Up Another Level of Control, if applicable
51
7 Remove the Diskette from Drive A: (after all needed set ups are complete)
51
Setting Up a RETIC File
51
1 Select the File
51
2 Type Entries from the Table of Expected Results
51
3 Verify Host Setting
51
4 Verify All Information
51
Sample analysis set up
52
Action limits
52
XB limits
52
Definitive flag limits
52
High/low flag limits
52
Laboratory Normal Ranges
52
Location list
52
Physician list
52
Display formats
52
Screen Labels
52
Parameter Selection
53
Reporting Units
53
Delete database
53
Delete host spooler
53
Clear printer spooler queue
53
Print options
53
Auto Print Format
53
Ticket Options
53
Spooler Priority
53
Graphics Options
53
Optional Printer
53
System set up
54
Shift
54
Reagents
54
Institution
54
Communication def
54
IQAP ID#
54
Set Date/Time
54
Supervisor Password
54
Optimize Hard Disk
54
QUALITY CONTROL
55
OBJECTIVES
55
When the subject is complete, you will be able to . . .
55
LATRON Primer and Control
55
5C Cell Control
55
Retic-C Cell Control
55
Interlaboratory Quality Assurance Program (IQAP)
55
Control File Management
56
Additional QC Checks
56
XB Analysis
56
LATRON PRIMER AND CONTROL
57
LATRON Primer
57
LATRON Control
57
Reviewing LATRON Control Results
58
On the Control Run Screen
58
Using Î Graph . . . a Control Run Screen Option
58
Using Review or Report, Latex . . . a Control Menu Option
58
Using Graphs . . . another Control Menu Option
58
1 Status for Proceeding
59
2 Run LATRON Primer
59
At the DMS
59
At the Diluter
59
Reviewing Primer Results at the DMS
59
3 Run LATRON Control
59
At the DMS
59
At the Diluter
60
Reviewing Control Results at the DMS
60
5C CELL CONTROL
61
Storage
61
Proper Handling
61
Prepare 5C Cell Controls according to the Package Insert Instructions
61
Stability
61
Open-Vial Stability
61
Indications of Instability
62
Running 5C Cell Control
62
Control Run Option
62
Recommendations for Running 5C Cell Controls
62
1 Getting the DMS Ready
63
If SELECT FUNCTION is displayed
63
If SELECT FUNCTION is not displayed
63
2 Prepare Controls according to the Package Insert
63
3 Run Controls in the same manner as Patient Samples
63
If using a HmX Hematology Analyzer with Autoloader
63
If using a HmX Hematology Analyzer
63
4 Review Results
64
If using a HmX Hematology Analyzer with Autoloader
64
For both the HmX Hematology Analyzer with Autoloader and the HmX Hematology Analyzer
64
RETIC-C CELL CONTROL
65
Storage
65
Proper Handling
65
Prepare according to the Package Insert Instructions
65
Stability
65
Open-Vial Stability
65
Indications of Instability
65
Running Retic-C Cell Control
66
Special Reagents Needed for Retic Analysis
66
1 Prepare Controls according to the Package Insert
67
2 Preparing the Control
67
3 Getting the DMS Ready
67
4 Run Controls in the Same Manner as Patient Samples
67
5 Review Results
67
REVIEWING CONTROL RESULTS
68
Using Control Menu Options to Review 5C Cell Control or Retic-C Results
68
On the Control Run Screen
68
Using Î Graph . . . a Control Run Screen Option
68
Using Review or Report, CBC/DIFF (or RETIC) . . . a Control Menu Option
68
Using Graphs . . . another Control Menu Option
68
TABLE OF QUALITY CONTROL MATERIALS
69
IQAP (Interlaboratory Quality Assurance Program)
69
CONTROL FILE MANAGEMENT
70
Existing Control Files Currently In Use
70
Reasons an Operator may need to Access a Control File that is Currently in Use
70
1 Select the File
71
2 Answer the Questions
71
3 Make the Changes
71
4 Verify All Changes
71
Existing Control Files No Longer In Use
72
Reason an Operator may need to Access an Existing File that is No Longer in Use
72
Management of Existing Control Files No Longer In Use . . . a Comparison
73
Review the Numeric Control Data and Graphs, if applicable
74
1 Locate the Control File
74
2 Review the Graphs
74
3 Review Numeric Data
74
Print / Download the Control File Data
74
Delete the Control File Data
74
1 Go to Control Review Screen
74
2 Delete the Control File Data
74
Set Up the New Lot Number of Control
74
Setting Up a 5C Cell Control File
75
1 Select the File
75
2 Upload the Assay Values
75
3 Type Manual Entries
75
4 Verify All Information
75
5 Set Up Another Level of Control, if applicable
75
6 Remove the Diskette from Drive A: (after all needed set ups are complete)
75
Setting Up a LATRON Control File
75
1 Select the File
75
2 Type Manual Entries
75
3 Verify All Information
75
Setting Up a RETIC File
76
1 Select the File
76
2 Type Entries from the Table of Expected Results
76
3 If the MRV and IRF Parameters Are Enabled
76
4 Verify All Information
76
Review the Numeric Control Data and Graphs, if applicable
77
1 Locate the Control File
77
2 Review the Graphs
77
3 Review Numeric Data
77
Print / Download the Control File Data
77
Changing the Existing File
77
1 Select the File
77
2 Answer the Questions
77
3 Upload the 5C Control Assay Values
78
4 Type Manual Entries
78
5 Verify All Information
78
6 Set Up Another Level of Control, if applicable
78
7 Remove the Diskette from Drive A: (after all needed set ups are complete)
78
Delete the Control File Data
78
1 Go to Control Review Screen
78
2 Delete the Control File Data
78
ADDITIONAL QC CHECKS
79
Mode-to-Mode Comparison
79
Linearity Checks
79
Diff Comparison
79
XB Analysis, a Quality Control Tool
80
RBC Indices
80
Target Values
80
Dr. Bull's Target Values
81
Getting Started
81
Enter Target Values and Action Limits
81
Turning the XB Option ON
81
Using the XB Analysis
81
Reviewing XB Data
82
Current XB Batch
82
XB Batch Means Option
83
XB Graphs
83
What Causes a Batch to go “Out-of-Limits”?
83
Troubleshooting when an XB Batch is “Out”
84
Consider a Change in the Patient Population
84
Checking for Non-Reportable Results
84
Consider an Instrument Problem Exists
84
Assessing the Situation
85
Identifying the Parameter Causing the Problem
85
Analysis of Involved Parameters
85
By Use of a Parameter Relationship Table
85
CALIBRATION
87
OBJECTIVES
87
When the subject is complete, you will be able to . . .
87
CALIBRATION
89
When to Calibrate
89
When to Verify Calibration
89
Preliminary Calibration Procedures
89
Procedures to be Completed Prior to Calibration
90
Calibration Procedure
90
CBC Calibration Screen
90
Terms and Formulas used in the CBC Calibration Procedure
91
MEAN
91
NEW CAL FACTOR
91
OLD CAL FACTOR
91
% CV
91
FACTOR % DIFF
91
DELTA DIFF
91
REFERENCE VALUES
91
SAMPLE ANALYSIS
93
OBJECTIVES
93
When the subject is complete, you will be able to . . .
93
Basics of Running a Sample
93
Cycling Options
93
Running Whole Blood Patient Samples
93
Processing a STAT Specimen (HmX Hematology Analyzer with Autoloader only)
93
Predilute Mode
93
Run Samples Screen
94
Retic Mode
94
Worklist (Optional)
94
SAMPLE HANDLING
95
Specimen Collection
95
Using Evacuated Collection Tubes
95
Using Microcollection Devices
95
Storage
95
BAR-CODE LABELING
95
Proper Placement of a Bar-Code Label on a Specimen Tube
96
Tube Adapters for Single Specimen, Rotary Cap-Pierce Instruments Only
96
CYCLING OPTIONS
97
F3-Run Options
97
Ê START PRIMARY
97
Ë SECONDARY
97
Ì PREDILUTE CBC
97
Í RETIC
97
Î DIFF ON/ OFF
97
Ï PURGE
97
à RINSE
97
Ñ STOP
97
F5-Optns
98
Ê XB
98
Ë CAP PIERCER: 10mm-13mm (Single Specimen, Rotary Cap-Pierce System Only)
98
Ì DB
98
Í Print
98
Î Host
98
Ï Display Only
99
à Operator
99
Ó B&W screen print
99
Ô Color screen print
99
RUNNING WHOLE BLOOD PATIENT SAMPLES (Using a HmX Hematology Analyzer)
101
Running Patient Samples in the Primary Mode
101
Scanning the Bar-Code Label on the Specimen Tube
101
Specimen Transport Overview
101
Running Patient Samples in the Secondary Mode
101
Steps for Changing the Carousel Slot
102
1 Getting the DMS Ready
103
If not using the Worklist
103
If using the Worklist
103
Finish DMS Preparation
103
2 Process the Sample
104
With Bar-Code Labels
104
Without Bar-Code Labels
104
3 When the Cycle is Complete
104
1 Getting the DMS Ready
105
2 Process the Sample
105
With Bar-Code Labels
105
Without Bar-Code Labels
105
3 When the Cycle is Complete
105
RUNNING WHOLE BLOOD PATIENT SAMPLES (Using a HmX Hematology Analyzer with Autoloader)
107
Running Patient Samples in the Primary Mode
107
Specimen Transport Overview
107
Running Patient Samples in the Secondary Mode
107
Running Whole Blood Samples in the Primary Mode
109
1 Getting the DMS Ready
109
If not using the Worklist
109
If using the Worklist
109
Finish DMS Preparation
109
2 Load Cassettes
109
3 When the Run is Complete
110
Interrupting Primary Mode Operation to Run a STAT
110
Running Whole Blood Samples in the Secondary Mode
110
1 Getting the DMS Ready
110
2 Process the Sample
110
3 When the Cycle is Complete
110
PREDILUTE MODE
111
For HmX Hematology Analyzer with Autoloader and HmX Hematology Analyzer
112
1 Getting the DMS Ready
112
If not using the Worklist
112
If using the Worklist
112
2 Make the Dilution
112
3 Process the Dilution
112
4 When the Cycle is Complete
112
RUN SAMPLES SCREEN
113
Screen Display
113
Run Samples Screen Areas
114
Demographics Area
114
CBC Result Area
114
Scatterplot
114
Condition Messages
116
Retic Mode
118
Sample Preparation Requirements
118
ReticPrep Reagent Kit
118
Equipment
118
Volume Requirements
118
Time Requirements
118
Reporting Retic Results
119
MRV and IRF Parameters
119
For HmX Hematology Analyzer with Autoloader and HmX Hematology Analyzer
120
1 Preparing the Sample
120
2 Getting the DMS Ready
120
3 Process the Sample
120
4 Reporting Retic Absolute Number
120
WORKLIST (Optional)
122
Status Messages
122
WL on the Status Line
122
Operator Response
122
PARTial ASPiration
122
3 Consecutive Partial Aspirations
122
NO MATCH
122
3 Consecutive NO MATCH Messages
123
NO READ
123
10 NO READ, NO MATCH, PART ASP
124
DATA REVIEW
125
OBJECTIVES
125
When the subject is complete, you will be able to . . .
125
Data Base Query
125
Basics of CBC Analysis
125
RBC Parameters
125
Plt Parameters
125
WBC Parameters
126
VCS Technology Basics
126
Flagging
126
DATA BASE QUERY
127
To Sort Sample Data
128
To View Sample Results
128
To Save Sample Data
129
To Edit Sample Data
129
To Output Sample Data: Print and Reprint / Transmit and Retransmit
130
To Print a Single Sample Data Set
130
To Batch Print or Transmit a Group of Samples
130
To Print a Condensed Line List
130
To Archive a Group of Stored Sample Results
131
To Review Archived Files
131
BASICS OF CBC ANALYSIS
133
Sample Dilutions
133
RBC Dilution
133
WBC Dilution
133
The Coulter Principle
134
Coincidence Correction
135
Count Periods
135
Thresholds
136
Histogram Development
136
Pulse Editing
136
Parameters Derived from a Histogram
137
Voting
137
Total Voteout
137
Single Count Period Voteout
137
RBC PARAMETERS
139
RBC Count
139
RBC Histogram
139
Characteristics of a Typical RBC Histogram
140
RDW (Red cell Distribution Width)
140
Flagging the RDW
141
Normal Distribution
141
Abnormal Distribution
141
Abnormal Distribution
141
Hemoglobin
142
Computed Parameters
142
PLATELET PARAMETERS
143
Sweep Flow
143
Plt Histogram
144
Characteristics of a Typical Platelet Histogram
145
Advantages of the Fitting Process
145
Criteria for Fit and No-Fit
146
Criteria for Fit and No-Fit (continued)
147
WBC PARAMETERS
149
WBC Count
149
WBC Differential
149
VCS TECHNOLOGY BASICS
151
Applications of the VCS Technology
151
Differential Analysis
151
Reticulocyte Analysis
151
Differential Analysis
152
Preparing the Sample
152
Analyzing the Sample
153
VCS Measurements
154
Volume
154
Conductivity
155
Opacity
155
Scatter
155
Simultaneous Analysis
156
VCS Cube
157
Cubes Within the Cube
158
Labeled Scatterplot
160
Discriminator Lines
160
DF1 View
160
DF2 View
160
DF3 View
160
Cube View
161
Reticulocyte Analysis
162
Typical Normal Retic Pattern - DF5 View
162
MRV and IRF Parameters
163
FLAGGING
164
Flagging Categories
164
Condition Messages
164
Suspect Messages
165
Operator-Definable Flags
166
Parameter Codes
166
BASIC TROUBLESHOOTING
167
OBJECTIVES
167
When the subject is complete, you will be able to . . .
167
Module and Component Identification
167
Sample Flow
167
Basic Troubleshooting Techniques
167
Replacement and Adjustment Procedures
168
MODULE AND COMPONENT IDENTIFICATION
169
CBC Module
169
BSV Module
170
Pump Module
171
Autoloader Module
172
Rotary Cap-Pierce Module
173
Flow Cell Module
174
Mixing Module
174
Main Diluter Module
175
SAMPLE FLOW
177
Aspiration
177
Primary Mode Aspiration on a HmX Hematology Analyzer
178
Primary Mode Aspiration on a HmX Hematology Analyzer - Step by Step
179
Primary Mode Aspiration on a HmX Hematology Analyzer with Autoloader
180
Primary Mode Aspiration on a HmX Hematology Analyzer with Autoloader - Step by Step
181
Piercing Needle Assembly
182
Draining the Baths
183
Preparing the CBC Sample
184
CBC Counting and Sensing
186
Preparing the Diff Sample
188
Diff Analysis
190
The Cycle Completes
191
BASIC TROUBLESHOOTING TECHNIQUES
193
How to Recognize an Instrument Problem
193
Three Steps to More Efficient Troubleshooting
193
Troubleshooting by Instrument Subsystems
194
Electronic Subsystem
194
Reagent Subsystem
194
Level Sensing
194
Replacement Procedures
194
Reagent Related Problems
194
Fluidic Subsystem
195
Key Components
195
Hydraulics
195
Pneumatics
195
Troubleshooting through Sample Flow
196
Troubleshooting using Special Procedures
196
Reproducibility Check
196
Carryover Check
197
SUMMARY PAGES
199
What are Summary Pages?
199
Purpose
199
Design
199
Presentation in This Training Guide
199
Routine Procedures
200
Retic Analysis
200
QC File Management
200
Replacement Procedures
200
CLEAN CYCLE SUMMARY
201
1 Perform the Clean Cycle
201
2 Let the Instrument Stand
201
3 Review Results
201
4 Perform Quality Control Checks
201
RUNNING LATRON Primer and Control Summary
203
1 Status for Proceeding
203
2 Run LATRON Primer
203
At the DMS
203
At the Diluter
203
Reviewing Primer Results at the DMS
203
3 Run LATRON Control
203
At the DMS
203
At the Diluter
203
Reviewing Control Results at the DMS
204
Running 5C Cell Control Summary
205
1 Getting the DMS Ready
205
If SELECT FUNCTION is displayed
205
If SELECT FUNCTION is not displayed
205
2 Prepare Controls according to the Package Insert
205
3 Run Controls in the same manner as Patient Samples
205
If using a HmX Hematology Analyzer with Autoloader
205
If using a HmX Hematology Analyzer
205
4 Review Results
205
If using a HmX Hematology Analyzer with Autoloader
205
For both the HmX Hematology Analyzer with Autoloader and the HmX Hematology Analyzer
206
Running Whole Blood Patient Samples Summary
207
Running Whole Blood Samples in the Primary Mode
207
1 Getting the DMS Ready
207
If not using the Worklist
207
If using the Worklist
207
Finish DMS Preparation
207
2 Load Cassettes
207
3 When the Run is Complete
208
Interrupting Primary Mode Operation to Run a STAT
208
Running Whole Blood Samples in the Secondary Mode
208
1 Getting the DMS Ready
208
2 Process the Sample
208
3 When the Cycle is Complete
208
Running Whole Blood Patient Samples in the Primary Mode Summary
209
1 Getting the DMS Ready
209
If not using the Worklist
209
If using the Worklist
209
Finish DMS Preparation
209
2 Process the Sample
210
With Bar-Code Labels
210
Without Bar-Code Labels
210
3 When the Cycle is Complete
210
1 Getting the DMS Ready
211
2 Process the Sample
211
With Bar-Code Labels
211
Without Bar-Code Labels
211
3 When the Cycle is Complete
211
Running Patient Samples in the Predilute Mode Summary
213
1 Getting the DMS Ready
213
If not using the Worklist
213
If using the Worklist
213
2 Make the Dilution
213
3 Process the Dilution
213
4 When the Cycle is Complete
213
DATA BASE QUERY QUICK REFERENCE GUIDE
215
To Sort Sample Data
215
To View Sample Results
215
To Save Sample Data
215
To Edit Sample Data
216
To Output Sample Data: Print and Reprint / Transmit and Retransmit
217
To Print a Single Sample Data Set
217
To Batch Print or Transmit a Group of Samples
217
To Print a Condensed Line List
217
To Archive a Group of Stored Sample Results
218
To Review Archived Files
218
STARTUP SUMMARY
219
1 Check Current Instrument Status
219
2 Perform Start Up
219
3 Review Results
219
4 Perform Quality Control Checks
219
SHUTDOWN SUMMARY
221
1 Perform Shut Down
221
2 Let the Instrument Stand
221
Running Retic-C Cell Control Summary
223
1 Prepare Controls according to the Package Insert
223
2 Preparing the Control
223
3 Getting the DMS Ready
223
4 Run Controls in the Same Manner as Patient Samples
223
5 Review Results
223
Running Samples in the Retic Mode Summary
225
1 Preparing the Sample
225
2 Getting the DMS Ready
225
3 Process the Sample
225
4 Reporting Retic Absolute Number
225
MAKING CHANGES TO A CONTROL FILE CURRENTLY IN USE
227
1 Select the File
227
2 Answer the Questions
227
3 Make the Changes
227
4 Verify All Changes
227
CONTROL FILE MANAGEMENT SUMMARY
229
Review the Numeric Control Data and Graphs, if applicable
229
1 Locate the Control File
229
2 Review the Graphs
229
3 Review Numeric Data
229
Print / Download the Control File Data
229
Delete the Control File Data
229
1 Go to Control Review Screen
229
2 Delete the Control File Data
229
Set Up the New Lot Number of Control
229
Setting Up a 5C Cell Control File
230
1 Select the File
230
2 Upload the Assay Values
230
3 Type Manual Entries
230
4 Verify All Information
230
5 Set Up Another Level of Control, if applicable
230
6 Remove the Diskette from Drive A: (after all needed set ups are complete)
230
Setting Up a LATRON Control File
230
1 Select the File
230
2 Type Manual Entries
230
3 Verify All Information
230
Setting Up a RETIC File
231
1 Select the File
231
2 Type Entries from the Table of Expected Results
231
3 If the MRV and IRF Parameters Are Enabled
231
4 Verify All Information
231
CONTROL FILE MANAGEMENT SUMMARY
233
Review the Numeric Control Data and Graphs, if applicable
233
1 Locate the Control File
233
2 Review the Graphs
233
3 Review Numeric Data
233
Print / Download the Control File Data
233
Changing the Existing File
233
1 Select the File
233
2 Answer the Questions
233
3 Upload the 5C Control Assay Values
234
4 Type Manual Entries
234
5 Verify All Information
234
6 Set Up Another Level of Control, if applicable
234
7 Remove the Diskette from Drive A: (after all needed set ups are complete)
234
Delete the Control File Data
234
1 Go to Control Review Screen
234
2 Delete the Control File Data
234
Changing Reagents Summary
235
1 Record the New Reagent Information
235
2 Replace the Reagent Container
235
3 Prime the Lines
235
Replacing a Waste Container Summary
237
1 Put the Instrument into Standby
237
2 Turn OFF the Main Power
237
3 Replace the Waste Container
237
4 Turn ON the Main Power
237
5 Put the Instrument into Ready
237
TRAINING CHECKLIST
239
OFFICIAL TRAINING DOCUMENTATION
239
Laboratory
239
Address
239
Phone
239
HmX Serial Number
239
Key Operator
239
Training Dates
239
Instructions
239
Checklist
239
Comments
240
TRADEMARKS
241
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Operator's Guide
130 pages
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Beckman Coulter COULTER HmX Specifications
General
Brand
Beckman Coulter
Model
COULTER HmX
Category
Laboratory Equipment
Language
English
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