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General| Software | CytExpert software for acquisition and analysis |
|---|---|
| Laser Options | 405 nm, 488 nm, 561 nm, 638 nm |
| Detection Channels | Up to 13 fluorescence channels |
| Dynamic Range | 7 decades |
| Lasers | 405 nm, 488 nm, 561 nm, 638 nm |
| Detectors | APDs |
| Forward Scatter (FSC) | Standard |
| Side Scatter (SSC) | Standard |
| Cell Size Range | 0.2 µm |
| Power Requirements | 100-240 V, 50/60 Hz |
Identifies and describes the three main components of the instrument: Fluid Containers, Cytometer, and Workstation.
Details the optical bench, WDMs, and optical fibers used for exciting, transmitting, and collecting optical signals.
Explains the fluidics system, including fluid containers/cubitainers and the fluidics module.
Describes the sample tube holder, sample probe, and wash station within the sample station.
Details the components of the plate loader, including the door, stage, and holder.
Details performance metrics like sensitivity, resolution, carryover, and throughput for CytoFLEX and LX models.
Details the Acquisition screen layout, including navigation, menus, controls, and status bar.
Describes the screen that appears when opening or creating a new compensation experiment.
Explains the Quality Control experiment screen and its associated report and navigation features.
Details system checks required before using the CytoFLEX or CytoFLEX LX flow cytometer.
Details the process of logging into the CytExpert software, including user management options.
Guides users on selecting between Semi-Automatic, Manual, and Plate Loader injection modes.
Explains how to switch to and use the Plate Loader sample injection mode.
Provides steps for initializing the instrument to prepare it for operation.
Details required materials and preparation processes for QC samples using daily fluorospheres.
Describes QC sample preparation steps when using the plate loader accessory.
Guides on importing lot-specific target values for QC fluorospheres for accurate results.
Provides instructions for performing QC experiments and collecting data.
Details QC data collection procedures when using the plate loader.
Explains how to review and confirm QC results, including criteria for passing QC.
Describes performing standardization using QC fluorospheres for application-specific settings.
Details managing standardization target values within the QC experiment for laser settings.
Guides on creating a new experiment or opening an existing one for data acquisition.
Details the process of creating experiments specifically for use with the plate loader.
Provides pre-run checks and procedures for loading samples and recording data.
Explains how to adjust settings for tubes, channels, labels, plots, gates, and lasers.
Covers analyzing data, adjusting gates, creating statistics, and exporting results.
Guides on preparing compensation samples and creating a compensation experiment.
Details creating compensation experiments specifically for use with the plate loader.
Explains importing single color data from other experiments for compensation calculations.
Covers manually adjusting compensation and importing/exporting compensation settings.
Explains calculating sample concentration using consumed volume or reference beads.
Covers general safety precautions and specific hazards associated with instrument use.
Details compliance with laser safety regulations and potential radiation exposure risks.
Explains the importance of laser warning labels and shields for personal safety.
Identifies and illustrates the locations of various hazard warning labels on the instrument.
Shows the location of biohazard labels on fluid containers and the instrument.
Lists common problems, probable causes, and corrective actions for the CytoFLEX flow cytometer.
Details procedures for backing up and restoring CytExpert data and configurations.
Specifies power source requirements and safety precautions for instrument operation.
Details the physical setup and connection of the Cytometer and workstation components.
Provides a step-by-step guide for installing the CytExpert software on a computer.
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