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Beckman Coulter Optima LE-80K User Manual

Beckman Coulter Optima LE-80K
52 pages
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Published by the Centrifuge Instrument Systems Development Center of Beckman Coulter, Inc., Palo Alto, California 94304
LLE7/8-IM-5
Instruction Manual
Optima
LE-80K
Preparative Ultracentrifuge
TM

Table of Contents

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Beckman Coulter Optima LE-80K Specifications

General IconGeneral
TypeUltracentrifuge
Maximum Speed80, 000 RPM
Temperature Range0°C to 40°C
Speed Control Accuracy± 20 rpm
Rotor TypesFixed-angle, swinging bucket
Control SystemMicroprocessor-controlled
Control PanelDigital display
ProgramsMultiple programs can be stored
Power Requirements200-240 VAC, 50/60 Hz
Refrigeration SystemCFC-free refrigeration

Summary

Safety Notice

Safety During Installation and/or Maintenance

Procedures and requirements for safe installation and maintenance of the instrument.

Electrical Safety

Guidelines and precautions related to electrical hazards and grounding for safe operation.

Safety Against Risk of Fire

Information on fuse protection and precautions against flammable or explosive vapors.

Mechanical Safety

Operational safety guidelines related to rotors, speed, and movement of the centrifuge.

Chemical and Biological Safety

Precautions for handling pathogenic, toxic, or radioactive materials and waste disposal.

Installation Requirements

Cautionary Note on Installation

Warning regarding unauthorized installation and its effect on warranty.

Preinstallation Requirements

Electrical Data and Power

Specifications for voltage, current, and wiring connections for power supply.

Electrical Wiring Connections

Table detailing wire insulation color, terminal, and symbol for connections.

Description

Diagnostic Messages

Information on red LED diagnostic messages, their blinking, and associated sounds.

Vacuum System Operation

Explanation of automatic vacuum activation, pressure LEDs, and venting procedures.

Temperature Control System

Explanation of the thermoelectric system and its control accuracy.

Overspeed System Safety

Description of the safety feature to prevent exceeding maximum rotor speed.

Run Preparation

Entering and Modifying Run Information

Process for entering and modifying run parameters, including editing mode.

Setting the Run Speed

Procedure for setting speed from 1000 to 80,000 rpm, including overspeed checks.

Activation Keys and Vacuum System

Using activation keys and the vacuum system for chamber evacuation.

Starting and Stopping Runs

Procedures for initiating a run with START and terminating with STOP.

Run Procedure

General Operation Safety Warnings

Critical warnings regarding flammable materials and leaning on the instrument.

Handling Hazardous Materials Safely

Precautions for operating with pathogenic, toxic, or radioactive materials.

Standard Operation Procedure

Step-by-step guide for performing a standard centrifugation run.

Programmed Operation Procedure

Steps for initiating a run using a pre-programmed setting.

Troubleshooting and Maintenance

Retrieving Samples After Power Failure

Detailed procedure for accessing the rotor and sample after a prolonged power outage.

Diagnostic Messages and Error Handling

Explanation of diagnostic messages, LEDs, and how to interpret them.

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